Diabetic Wound Healing Biosurfactants Dialkyl Alginate Cream on TNF-α TGF-β Expression, Reepithelization, and Collagenization

  • Cut Raihanah Universitas Ahmad Dahlan
  • Nurul Mahyani Universitas Ahmad Dahlan
  • Kintoko Kintoko Universitas Ahmad Dahlan

Abstract

Diabetic wound healing is delayed by many factors, including high TNF-α expression and low TGF-β expression which can affect the formation of new epithelial tissue and collagen as the main goal of the wound healing process. One of the diabetic wound healing agent is biosurfactant dialkyl alginate where so far its use in cream form for diabetic wound has never been reported. This study aimed to determine TNF-α, TGF-β, reepithelization and the collagenization of biosurfactant dialkyl alginate cream in diabetic biopsy wounds in STZ-induced rat. Biosurfactant dialkyl alginate was made in cream form and applied to biopsy wounds on the backs of rat twice a day for 9 days. Observation of TNF-α and TGF-β expression were performed by immunohistochemical staining, while epithelial and collagen with staining HE and Mallory. The results showed that the biosurfactant dialkyl alginate cream had an activity to decrease TNF-α expression, increase TGF-β expression and reepithelization but did not have any significant activity on collagenization. These results suggest that the biosurfactant dialkyl alginate cream can accelerate the healing of diabetic wound.

References

1. Kavitha KV. Choice of wound care in diabetic foot ulcer: A practical approach. World Journal of Diabetes. 2014.5(4):546.

2. Xu F, Zhang C, Graves DT. Abnormal cell responses and role of TNF- α in impaired diabetic wound healing. BioMed Research International. 2013.2013:1–9.

3. Kristianto H, Nurachmah E, Gayatri D. Peningkatan ekspresi transforming growth factor beta 1 (TGF β1) pada luka diabetes melitus melalui balutan modern. Jurnal Keperawatan Indonesia. 13(1):6.

4. Ramirez H, Patel SB, Pastar I. The role of TGF-β signaling in wound epithelialization. Advances in Wound Care. 2014.3(7):482–91.

5. Pakyari M, Farrokhi A, Maharlooei MK, Ghahary A. Critical role of transforming growth factor beta in different phases of wound healing. Advances in Wound Care. 2013.2(5):215–24.

6. DeClue CE, Shornick LP. The cytokine milieu of diabetic wounds. Diabetes Management. 2015.5(6):525–37.

7. McKelvey K, Xue M, Whitmont K, Shen K, Cooper A, Jackson C. Potential anti-inflammatory treatments for chronic wounds. Wound Practice & Research: Journal of the Australian Wound Management Association. 2012.20(2):86–9.

8. Cukjati D, Reberšek S, Miklavčič D. A reliable method of determining wound healing rate. Medical & Biological Engineering & Computing. 2001.39(2):263–71.

9. Prasetyono TOH. General concept of wound healing , revisited. Med J Indones. 2009.18(3):208–216.

10. Broughton G, Janis JE, Attinger CE. Wound healing: An overview. Plastic and Reconstructive Surgery. 2006.117(7 SUPPL.):1–32.

11. Percival SL, Mayer D, Malone M, Swanson T, Gibson D, Schultz G. Surfactants and their role in wound cleansing and biofilm management. Journal of Wound Care. 2017.26(11):680–90.

12. Zhao G, Usui ML, Lippman SI, James GA, Stewart PS, Fleckman P, et al. Biofilms and inflammation in chronic wounds. Advances in Wound Care. 2013.2(7):389–399.

13. Diegelmann, R. F., and Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Frontiers in Bioscience. 2004.9(1–3):283.

14. Sambanthamoorthy K, Feng X, Patel R, Patel S, Paranavitana C. Antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens. BMC Microbiology. 2014.14(1):197.

15. Banat IM, De Rienzo MAD, Quinn GA. Microbial biofilms: biosurfactants as antibiofilm agents. Applied microbiology and Biotechnology. 2014.98(24):9915–9929.

16. Gupta S, Raghuwanshi N, Varshney R, Banat IM, Srivastava AK, Pruthi PA, et al. Accelerated in vivo wound healing evaluation of microbial glycolipid containing ointment as a transdermal substitute. Biomedicine & Pharmacotherapy. 2017.94:1186–96.

17. Piljac A, Stipcevic T, Piljac-Zegarac J, Piljac G. Successful treatment of chronic decubitus ulcer with 0.1% dirhamnolipid ointment. Journal of cutaneous medicine and surgery. 2008.12(3):142–146.

18. Stipcevic T, Piljac A, Piljac G. Enhanced healing of full-thickness burn wounds using di-rhamnolipid. Burns. 2006.32(1):24–34.

19. Zhou R, Shi X, Gao Y, Cai N, Jiang Z, Xu X. Anti-inflammatory activity of guluronate oligosaccharides obtained by oxidative degradation from alginate in lipopolysaccharide-activated murine macrophage raw 264.7 cells. Journal of Agricultural and Food Chemistry. 2015.63(1):160–8.

20. Hajiali H, Summa M, Russo D, Armirotti A, Brunetti V, Bertorelli R, et al. Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing. Journal of Materials Chemistry B. 2016.4(9):1686–95.

21. Laurienzo P. Marine polysaccharides in pharmaceutical applications: An overview. Marine Drugs. 2010.8(9):2435–65.

22. Razafindralambo H, Blecker C, Paquot M. Carbohydrate-based surfactants: structure-activity relationships. Advances in Chemical Engineering; 2012.215–228 p.

23. Oktavia MD, Ayu SK, Halim A. Pengaruh basis krim terhadap penetrasi kloramfenikol menggunakan kulit mencit. Open Journal Systems. 2012.4(1):42–9.

24. Rodríguez-Luna A, Talero E, Terencio M, González-Rodríguez M, Rabasco A, de los Reyes C, et al. Topical application of glycolipids from isochrysis galbana prevents epidermal hyperplasia in mice. Marine Drugs. 2018.16(2):1–19.

25. Kintoko K, Karimatulhajj H, Elfasyari TY, Ihsan EA, Putra TA, Hariadi P, et al. Effect of diabetes condition on topical treatment of binahong leaf fraction in wound healing process. Majalah Obat Tradisional. 2017.22:103.

26. Dipahayu D, Soeratri W, Agil M. Formulasi krim antioksidan ekstrak etanol daun ubi jalar ungu (Ipomoea batatas (l.) lamk) sebagai anti aging. Pharmaceutical Sciences and Research. 2014.1(3):166–79.
Published
2019-04-24
How to Cite
RAIHANAH, Cut; MAHYANI, Nurul; KINTOKO, Kintoko. Diabetic Wound Healing Biosurfactants Dialkyl Alginate Cream on TNF-α TGF-β Expression, Reepithelization, and Collagenization. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 1, p. 72-80, apr. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/688>. Date accessed: 17 sep. 2019. doi: https://doi.org/10.35814/jifi.v17i1.688.
Section
Articles