Eugenol Effect of Clove Flower (Syzygium Aromaticum) Isolate on Histology Pancreas of Diabetic Rat

  • Sinta Wahyu Utami Fakultas Kedokteran, Fakultas MIPA Universitas Mataram
  • I Made Sudarma Fakultas Kedokteran, Fakultas MIPA Universitas Mataram
  • Candra Dwipayana Hamdin Fakultas Kedokteran, Fakultas MIPA Universitas Mataram
DOI: https://doi.org/10.35814/jifi.v17i2.694

Abstract

Diabetes mellitus is disease with a high prevalence rate and continuously increase in Indonesia. So some researchers began to glance at natural rescource for prevention and treatment of diabetes mellitus. Clove Flower (Syzygium aromaticum) contains eugenol that can chemically lower blood sugar levels. The aimed of this research is to know the effect  of eugenol on pancreas histology of diabetic rats. Clove flowers was extracted used maseration method  use n-hexan and eugenol was isolated tested with TLC. Alloxan-induced rats were dosed 125 mg / kg BW IP and then the eugenol was administered in  dosage 10 mg / kg BW compared with glibenclamid 1,35 mg /Kg BW orally for 14 days. Observation of pancreatic histology results were made pancreatic preparations using Hematoxilin Eosin (HE) staining. The results showed that alloxan-induced rats had pancreatic cell damage. How ever, after being treated with Glibenclamide and Eugenol showed the improvement of damage organs. Giving the eugenol at a dose of 15 mg / kg BW showed the most significant improvement.

References

1.Wahyuni, R., Arsunan, A.A.,Zulkifli Abdullah A. 2013. Factor Releted to Anciety Levels In Patients With Diabete Mellitus Type 2 Bhayangkara Andi Mappang Audang Hospital. Makassar : Universitas Hasanuddin.

2. Dipiro, J. T., Talbert, R. L., Yee, G. C., Matzke, G. R., Wells, B. G., and Posey, L. M., 2015. Pharmacotherapy : Patophysiologic Approach. 9th Edition, New York: MC Graw Hill, hal. 212-210.

3. Srinivasan, S., Sathis, G., Jayanthi, M., Muthukumaran, J., Muruganthan, U., Ramachandran, V., 2013. Ameliorating effect of eugenol on hyperglycemia by attenuating the key enzymes of glucose metabolism in streptozotocin-induced diabetic rats. Mol Cell Biochem., Vol. 385 No. 2, pp. 159-168. Diakses dari https://www.ncbi.nlm.nih.gov/pubmed/24078031, pada tanggal 21 Januari 2019.

4. Cho, N. Han., Whiting, D., Forouhi, N., Guariguata, L., Hambleton, I., Li, R., Majeed, A., Mbanya, J. C., Montoya, P. A., Motala, A., Narayan, K. M. V., , Ramachandran, A., Rathmann, W., Roglic. G., Shaw, J., Silink, M., and Zhang , P. Internasional Diabetes Federation Diabetes Atlas. 7Th Edition. Diakses dari www.idf.org/e-library/epidemiology-research/diabetes-atlas/134-idf-diabetes-atlas-8th-edition.html, pada tanggal 27 Desember 2017.

5. Sidabutar, D. M., Kairupan, C. F., dan Meilany, D., 2016. Pengaruh pemberian ekstrak daun cengkeh (Syzygium aromaticum) terhadap gambaran histopatologik hati tikus wistar yang diberikan parasetamol dosis toksik. Jurnal e-Biomedik (eBm)., Vol. 4 No. 1, pp. 1-4. Diakses dari https://ejournal.unsrat.ac.id/index.php/ebiomedik/article/view/12224, 21 Januari 2019.

6. Abrao, P.H.O., Pizi, R.B., Souza, T.B.d., Silva, N.C., and Fregnan, A.M. Synthesis And Biological Evaluation Of New Eugenol Mannich Bases As Promising Antifungal Agent. Diakses dari http: Onlinelibrary.wiley.com//10.111/cbdd.12345/full, Pada tanggal 16 Januari 2018.

7. Mnafgui, K., Kaanich, F., Derbali, A., Hamden, K., Derbali, F., Slama, S., Allouche, N., and Elfeki, A., 2013. Inhibition of key enzymes related to diabetes and hypertension by Eugenol Invitro and in Alloxan-induced diabetic rats. Arch Physiol Biochem., Vol. 119 No. 5, pp. 1-9 Diakses dari https://www.tandfonline.com/doi/abs/10.3109/13813455.2013.822521, pada tanggal 22 Desember 2018.

8. Sudarma, M., Ulfa, M., Sarkono, 2009. Sintesis 4-Allil-2-metoksi-6-aminofenol dari Senyawa Bahan Alam Eugenol. Indo. J. Chem., Vol. 9 No. 1, pp. 84-88. Diakses dari https://journal.ugm.ac.id/ijc/article/viewFile/21566/14271, pada tanggal 21 Januari 2019.

9. Risayanti, Made Dwi, 2017, Penambahan Basa Organik ( Heksamina) dan Basa Anorganik (Natrium Bikarbonat) Pada Reaksi Sulfonasi Eugenol Hasil Isolasi Minyak Cengkeh, Skripsi, Mataram : Universitas Mataram.

10. Azhari, B., Luliana, sri., Robiyanto, 2017. Uji Aktivitas Antihiperkolesterolemia Ekstrak AirBuah Belimbing Wuluh (Averrhoa bilimbi Linn.) Pada Permodelan Tikus Jantan Galur Wistar Hiperkolesterolemia. Traditional Medicine Journal. Vol. 4, No 1, pp : 57-62, Diakses dari pada tanggal 20 Januari 2019.

11. Akrom, Harjani, P.D., dan Armansyah. Efek Hipoglikemik Ekstrak Etanol Umbi Ketela Rambat (Ipomoea Batatas P) (Eeukr) Pada Mencit Swiss Yang Diinduksi Aloksan. Pharmaciana, Vol.4, No.1, p : 65-76. Diakses dari http://eprints.uad.ac.id/8612/, pada Departemen kesehatan Republik Indonesia. 2008. Keputusan Menteri Kesehatan Repubik Indonesia Nomor 261/MENKES/SK/IV/2009 tentang Farmakope Herbal Indonesia Edisi Pertama. Jakarta : Departemen Kesehatan Repubik Indonesia.

12. Muliasari, H., Hamdin, C.D., Ihsan, M., 2017. Histologi Pankreas Tikus Diabetes Setelah Pemberian Suspensi Biji Buah Makasar (Brucea Javanica (L.) Merr). BioWallacea Jurnal Ilmiah Ilmu Biologi., Vol.3 No. 2, pp 88-93. Diakses dari http://eprints.unram.ac.id/10077/ , pada tanggal 22 Desember 2018.

13. Lanzen, S., 2008. The Mechanism of alloxan and Streptozotocin Induced Diabetes. Diabetologia, Vol. 52. No. 2, pp. 226-216.

14. Krentz, A.J., and Bailey, C. J., 2005. Oral Antidiabetic Agents Current Role in Type 2 Diabetes Mellitus. UK : Aston University, Hal. 398-390.
Published
2019-10-29
How to Cite
UTAMI, Sinta Wahyu; SUDARMA, I Made; HAMDIN, Candra Dwipayana. Eugenol Effect of Clove Flower (Syzygium Aromaticum) Isolate on Histology Pancreas of Diabetic Rat. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 2, p. 160-163, oct. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/694>. Date accessed: 26 dec. 2024. doi: https://doi.org/10.35814/jifi.v17i2.694.
Citation Formats
Issue
Vol 17 No 2 (2019): JIFI
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.