Phytochemical Screening and Potential Antioxidant Activity of Ethanol Ekstract of Green Leaves and Red Leaves Kastuba

  • Baiq Sopiah Universitas Mataram
  • Handa Muliasari Universitas Mataram
  • Emmy Yuanita Universitas Mataram
DOI: https://doi.org/10.35814/jifi.v17i1.698

Abstract

Free radicals are an atom or molecule that has unpaired electron in its outer orbital that causes maked this reactive and unstable molecule. Free radicals when exposed continuously in the body are believed to cause several diseases include atherosclorosis, ischemia, cancer and premature aging therefore, antioxidant compounds is needed to inhibit the formation of free radicals. Kastuba (Euphorbia Pulcherrima Willd.) is one of the plants that has the potential as a natural antioxidant. The aim of this study was determining the content of active chemical compounds and free radical inhibitory potential of kastuba leaves ethanol ekstract.The procedures of this study were material collection, plant determination, simplicia production, extraction, phytochemical screening and qualitative free radical inhibition test with TLC (Thin Layer Chromatography). Phytochemical screening aim to determine the chemical compounds component of kastuba leaves ethanol extract including flavonoids, tannins and terpenoid test. The potential of free radical inhibition was carried out qualitatively with the TLC,  DPPH (1,1-diphenyl-2-picrylhydrazyl) spray reagent. The results showed that kastuba leaves ethanol extract for both green leaves and red leaves contained flavonoids, tannins and terpenoids, and both leaves had the potential as free radical inhibition that indicated by yellow spots on TLC after DPPH reaction spray.

References

1. Kikuzaki, H., Hisamoto, M., Hirose, K., Akiyama, K., and Taniguchi, H. 2009. Antioxidant Properties of Ferulic Acid its Related Compounds. Journal of Agricultura. Vol. 50. Diakses dari https://pubs.acs.org/ doi/pdf/10.1021/ jf011348w pada tanggal 18 September 2018.

2. Aseervatham, G.S.B., Sivasudha, T., Jeyadevi, R., and Arul,A., D. 2013. Environmental Factors and Unhealthy Lifestyle Influence Oxidative Stress in Humans-An Overview. Journal springer. Vol. 20. Diakses dari https://link.springer.com/article/10.1007%2Fs11356-013-1748-0 pada tanggal 18 Desember 2018.

3. Kunwar, A., and Priyadarsini K.I. 2011. Free Radicals, Oxidatives Stress And Infortance of Antioxidant in
Human Health. J. Med Allied Sci. Vol. 1(2). Diakses dari https://www.google.com/search?client=firefox-b-ab&q=Free+Radicals%2C+Oxidatives+stress+And+Infortance+Of+Antioxidant+in+Human+Health pada tanggal 18 Desember 2018.

4. Islam, M. A., Toherstensen., and Clarke. 2015. Poinsettia (Euphorbia Pulcherrima Willd ex. Klotzsch). Article in Methods in molecular biology (Clifton, N.J.). Vol. 1224. Diakses dari https://link.springer.com/ protocol/10.1007%2F978-1-4939-1658-0_27 pada tanggal 18 Desember 2018.

5. Yakubu, A.I and Mukhtar, M.D. 2011. In Vitro Antimicrobial Activity of Some Phytochemical Fraction of Euphorbia Pulcherrima L. (Poinsettia). Journal of Medicinal Plant Research. Vol. 5(12). Diakses dari http://www.academicjournals.org/JMPR. pada tanggal 9 januari 2019.

6. Sunarni, T., Pramono, S., dan Asmah R. 2007. Flavonoid Antioksidan penangkal radikal dari Daun Kepel (Stelechocarpus burahol (B.I). Majalah Farmasi Indonesia. Vol. 18(3) diakses dari https://download.portalgaruda.org.article pada tanggal 9 januari 2019

7. Anief, M. 2010. Ilmu Meracik Obat. Yogyakarta: UGM Press, hal. 34-36.

8. Marliana, S. D., Suryanti, V., dan Suyono. 2005. Skrining Fitokimia dan Analisis Kromatografi lapis Tipis Komponen Kimia Buah labu silam (Sechium edule Jacq. Swartz dalam Ekstrak etanol. Jurnal Universitas Sebelas Maret. Diakses dari biosains.mipa.uns.ac.id/F/F0301/F030106.pdf pada tanggal 7 januari 2019.

9. Banu, R.H.,Nagarajan, N. 2014. TLC and HPTLC Fingerprinting of Leaf Extracts of Wedelia chinensis (Osbeck) Merril. Journal of Pharconognosy and Phytochemistry. Vol. 2 No. 6. Diakses dari www.phytojournal.com/archives/2014/vol2issue6 pada tanggal 7 Januari 2019.

10. Ningsih, D.r., Zusfahair dan Dwi, K. 2016. Identifikasi Senyawa Metabolit Sekunder serta Uji Aktivitas Ekstrak Daun Sirsak Sebagai Antibakteri. Jurnal Universitas Jenderal Soedirman. Diakses dari https://ojs.jmolekul.com/ojs/index.php/jm/article/viewFile/199/201 pada tanggal 7 januari 2019.

11. Ghosal, M. and Mandal, P. 2012. Phytomical Screening and Antioxidant Activities Of Two Selected “Bihi” Fruits Used as Vegetables In Darjeeling Himalaya. Int. Jurnal Phar.Sci. Vol. 4 No. 2. Diakses dari https://pdfs.semanticscholar.org/a9d7/d699b8580e1fe654d0de5e671d5e20786a4d.pdf pada tanggal 9 januari 2019.

12. Gandjar, I.G., Rohman, A. 2007. Kimia Farmasi Analisis. Yogjakarta : Pustaka Pelajar, hal. 253-254, 353-360.

13. Banu, R.H., Nagarajan, N. 2014. TLC and HPTLC Fingerprinting of Leaf Extracts of Wedelia chinensis (Osbeck) Merrill. Journal of Pharconognosy and Phytochemistry. Vol. 2 No 6. Diakses dari www.phytojournal.com/archives/2014/vol2issue6 pada tanggal 7 Januari 2019.

14. Lung, J.K.S., dan Dika P.D. 2017. Uji Aktivitas Antioksidan Vitamin A,C,E dengan metode DPPH. Jurnal Universitas Padjajaran. Diakses dari lib.ui.ac.id/file?file=pdf/abstrak-77086.pdf pada tanggal 7 januari 2019.

15. Hakim, A. 2016. Menigkatkan Kualitas Pembelajaran Kimia Bahan Alam melalui Praktikum. Mataram : Penerbit Arga Puji Press, hal. 48-49.
Published
2019-04-24
How to Cite
SOPIAH, Baiq; MULIASARI, Handa; YUANITA, Emmy. Phytochemical Screening and Potential Antioxidant Activity of Ethanol Ekstract of Green Leaves and Red Leaves Kastuba. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 1, p. 27-33, apr. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/698>. Date accessed: 16 apr. 2024. doi: https://doi.org/10.35814/jifi.v17i1.698.
Citation Formats
Issue
Vol 17 No 1 (2019): JIFI
Section
Articles

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.