The In Vitro and In Silico Study of n’-benzoylisonicotinohydrazide as Antituberculosis Candidate

  • Ruswanto Ruswanto STIKes BTH Tasikmalaya
  • Nur Rahayuningsih Dept. of Pharmacy, Bakti Tunas Husada Health Science College
  • Nur Laeli Dwi Hidayati Dept. of Pharmacy, Bakti Tunas Husada Health Science College
  • Ginna Sri Nuryani Dept. of Pharmacy, Bakti Tunas Husada Health Science College
  • Richa Mardianingrum Dept. of Pharmacy, Bakti Tunas Husada Health Science College

Abstract

There have been in silico and in vitro studies of N-Benzoylisonicotinohydrazide derivatives as antituberculosis candidates. The aims of this research were  determined that the N'-benzoylisonicotinohydrazide derivatives could inhibit the activity of gram-positive, gram-negative and Mycobacterium tuberculosis, as well as having good interactions with Enoyl-Acyl Carrier Protein Reductase from Mycobacterium Tuberculosis. From the in vitro test it was found that the N'-benzoylisonicotinohydrazide compound had a Minimum Inhibitor Concentration (MIC) of 0.33 µg / ml against the Basillus subtilis, while the Minimum Inhibitor Concentration (MIC) of N '- (2-chlorobenzoyl) isonicotinohydrazide against Mycobacterium tuberculosis (H37Rv) was 3.125 µg / ml. From the in silico study, it was found that the binding affinity value between N '- (2-chlorobenzoyl) isonicotinohydrazide enzyme Enoyl-Acyl Carrier Protein Reductase (2X23) had the smallest binding affinity so that it could be predicted that it had a stable interaction than other compounds so that the N '- (2-chlorobenzoyl) isonicotinohydrazide could be used as a more potent antituberculosis candidate.

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Published
2019-10-29
How to Cite
RUSWANTO, Ruswanto et al. The In Vitro and In Silico Study of n’-benzoylisonicotinohydrazide as Antituberculosis Candidate. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 2, p. 218-226, oct. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/703>. Date accessed: 18 nov. 2019. doi: https://doi.org/10.35814/jifi.v17i2.703.
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