Anti-inflammatory Activity of Crude Fucoidan from Sargassum crassifolium through Inhibition of ICAM-1 and VCAM-1 in Lipopolysaccharide-Induced Raw 264.7 Cell

  • Ayu Werawati Universitas Pancasila
  • Kusmardi Kusmardi Universitas Indonesia


Inflammation is the response of vascular tissue to infection and damaged tissue to remove agents that cause inflammation. Macrophages are the dominant cells in the inflammatory reaction. Adhesion of leukocytes to vascular endothelium is a main feature of the inflammatory process. The presence of adhesion molecules, such as ICAM-1 and VCAM-1, can lead to adhesion of monocytes and lymphocytes to endothelial cells via bonding adhesion molecules to endothelial cells. Crude fukoidan derived from Sargassum crassifolium taken from Garut waters and extracted with dilute HCl, tested for anti-inflammatory activity through inhibition of ICAM-1 and VCAM-1 using  lipopolysaccharide-induced RAW 264.7 cell models. The viability test was carried out in the concentration range of 0.49-1000 µg / mL. The pattern of inhibition of cell proliferation is influenced by concentration. The highest concentration that caused cell viability of 80% was the concentration of 86.46μg / ml. Crude fukoidan reduced ICAM-1 and VCAM-1 levels at concentrations of 50 and 25 μg / ml.. Percent inhibition of ICAM-1 at 50 µg / mL concentration  was 61.04% compared to control and 74.26% at 25 µg / mL concentration. While the percent inhibition of VCAM-1 a


1. Pakidi CS, Suwoyo HS. Potensi dan Pemanfaatan Bahan Aktif Alga Cokelat Sargassum sp. Octopus. 2016;5(2):488–98.

2. Nurul Falah R. Preparasi dan karakterisasi nanopartikelcrude fukoidan ekstrak rumput laut coklat (Sargassum polycystum) dan uji aktivitas antiplatelet secara in vitro. Prep dan karakterisasi nanopartikelcrude fukoidan ekstrak rumput laut coklat (Sargassum polycystum) dan uji Akt antiplatelet secara Vitr. 2017;

3. Asih S. Uji Aktivitas Antiplatelet Fukoidan dari Rumput Laut Coklat (Sargassum crassifoilum) yang Diperoleh Dari Peraian Garut Dengan Waktu Panen Yang Berbeda. 2018;

4. Udani J. The Potential Use of Fucoidans from Brown Seaweed as a Dietary Supplement. J Nutr Food Sci [Internet]. 2012;02(10):1–6. Available from:

5. Cumashi A, Ushakova NA, Preobrazhenskaya ME, D’Incecco A, Piccoli A, Totani L, et al. A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology. 2007;17(5):541–52.

6. Phull A, Majid M, Haq I, Rashid M, Ja S. International Journal of Biological Macromolecules In vitro and in vivo evaluation of anti-arthritic , antioxidant efficacy of fucoidan from Undaria pinnatifida ( Harvey ) Suringar. Int J Biol Macromol [Internet]. 2017;97:468–80. Available from:

7. Simpi CC, Nagathan C V, Karajgi SR, Kalyane N V. Evaluation of marine brown algae Sargassum ilicifolium extract for analgesic and anti-inflammatory activity. 2013;5(3).

8. Juliana A Purukan (Universitas Pancasila), Kusmardi, Bambang Ponco Priosoeryanto, Dian Ratik Laksmitawati S. Perbandingan profil lipid pada tikus putih jantan yang diberi crude fucoidan yang diinduksi diet tinggi lemak. 2019;

9. Joung EJ, Gwon WG, Shin T, Jung BM, Choi JS, Kim HR. Anti-inflammatory action of the ethanolic extract from Sargassum serratifolium on lipopolysaccharide-stimulated mouse peritoneal macrophages and identification of active components. J Appl Phycol. 2017;29(1):563–73.

10. Fitton JH, Stringer DN, Karpiniec SS. Therapies from fucoidan: An update. Mar Drugs. 2015;13(9):5920–46.

11. Fletcher HR, Biller P, Ross AB, Adams JMM. The seasonal variation of fucoidan within three species of brown macroalgae. Algal Res [Internet]. 2017;22:79–86. Available from:

12. Klingenberg R, Hansson GK. Treating inflammation in atherosclerotic cardiovascular disease: Emerging therapies. Eur Heart J. 2009;30(23):2838–44.

13. Jones DP, True HD, Patel J. Leukocyte Trafficking in Cardiovascular Disease: Insights from Experimental Models. Mediators of Inflammation. 2017.

14. Laksmitawati DR, Prasanti AP, Larasinta N, Syauta GA, Hilda R, Ramadaniati HU, et al. Anti-Inflammatory Potential of Gandarusa (<I>Gendarussa vulgaris</I> Nees) and Soursoup (<I>Annona muricata</I> L) Extracts in LPS Stimulated-Macrophage Cell (RAW264.7). J Nat Remedies [Internet]. 2016;16(2):73. Available from:

15. Sandhiutami NMD, Moordiani M, Laksmitawati DR, Fauziah N, Maesaroh M, Widowati W. In vitro assesment of anti-inflammatory activities of coumarin and indonesian cassia extract in RAW264.7 murine macrophage cell line. Iran J Basic Med Sci. 2017;20(1):99–106.

16. Aydin S. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides [Internet]. 2015;72:4–15. Available from:

17. Wang X, Pei L, Liu H, Qv K, Xian W, Liu J, et al. Fucoidan Attenuates Atherosclerosis in LDLR-/- Mice Through Inhibition of Inflammation and Oxidative Stress. Int J Clin Exp Pathol. 2016;9(7):6896–904.

18. Satyarsa ABS, Education M, Program S. Potential of Fucoidan From Brown Seaweeds ( Sargassum sp . ) as Innovation Therapy on Breast Cancer Studi Pustaka : Potensi Fucoidan dari Rumput Laut Coklat ( Sargassum sp . ) sebagai Inovasi Terapi pada Kanker Payudara. 2019;2(3):909–19.

19. Yang JW, Yoon SY, Oh SJ, Kim SK, Kang KW. Bifunctional effects of fucoidan on the expression of inducible nitric oxide synthase. Biochem Biophys Res Commun. 2006;346(1):345–50.
How to Cite
WERAWATI, Ayu; KUSMARDI, Kusmardi. Anti-inflammatory Activity of Crude Fucoidan from Sargassum crassifolium through Inhibition of ICAM-1 and VCAM-1 in Lipopolysaccharide-Induced Raw 264.7 Cell. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 1, p. 95-102, apr. 2019. ISSN 2614-6495. Available at: <>. Date accessed: 17 july 2019.