Separation Selectivity of Isoniazid and Asetilisoniazid in Human Plasma In-vitro by High Performance Chromatography

  • Novi Yantih UNIVERSITAS PANCASILA
  • Yahdiana Harahap Fakultas Farmasi Universitas Indonesia
  • Wahono Sumaryono Fakultas Farmasi Universitas Pancasila
  • Rianto Setiabudy Fakultas Kedokteran Universitas Indonesia
  • Lestari Rahayu Fakultas Farmasi Universitas Pancasila
DOI: https://doi.org/10.35814/jifi.v17i2.717

Abstract

Bioanalysis method is needed to pharmacokinetic study of INH as antituberculosis. The main problem is INH structure is similar to that of acetyl isoniazid (AcINH) as its metabolite. Therefore, a selective separation method is needed to separate the INH from its metabolite and matrix. The aim of this study was to test the selectivity of separation method of INH and AcINH in human plasma in-vitro by high performance liquid chromatography (HPLC). The HPLC system used a reversed phase with UV detector and hexane sulphonate as an ion counter. The optimum conditions was obtained by using C18 as stationary phase, hexane sulphonate (pH 2.47)-methanol (65:35) as mobile phase, with flow rate of 1mL/min, and UV detector at wavelength of 265nm. The selectivity of method separation was indicated by a resolution value of ≥ 1.5. The tailing factor for INH and AcINH were 1.297, dan 1.912, respectively. The k values ware ​​less than 10 and N values ​​were greater than 5000 indicate good separation efficiency. The developing of HPLC was a selective for separating of INH and AcINH in human plasma in-vitro.

References

1. WHO (World Health Organization). 2010. Treatment of Tuberculosis: Guidelines. 4th. Geneva: World Health Organization; Stop TB Initiative (World Health Organization).

2. Klein DJ, S Boukouvala, EM McDonagh, SR Shuldiner, N Laurieri, CF Thorn, RB Altman, TE Klein. PharmGKB summary: isoniazid pathway, pharmacokinetics. Pharmacogenet Genomics; 2016. 26(9): 436–444.

3. Teixeira RLF, RG Morato, PH Cabello, LMK Muniz, ASR Moreira, AL Kritski, FCQ Mello, PN Suffys, AB Miranda,
dan AR Santos. Genetic polymorphisms of NAT2, CYP2E1 and GST enzymes and the occurrence of antituberculosis drug-induced hepatitis in brazilian TB patients, Mem Inst Oswaldo Cruz. 2011. 106 (6). Rio de Janeiro. Sept. ISSN 0074-0276. http://dx.doi.org/10.1590/S0074-02762011000600011.

4. Stella. Optimasi dan validasi metode analisis isoniazid dan pirazinamid dalam tablet 4 fixed dosecombination(4FDC) dan plasma in vitro secara kromatografi cair kinerja tinggi [Skripsi] FMIPA UI. 2011.

5. Madhavi R, Mohana KA, Shobha RG, Mounika D. Isoniazid: A Review of Analytical Methods. Asian J. Pharm. Ana. 2015. 5(1):41-5.

6. Bhandari R, IP Kaur. A sensitive HPLC method for determination of isoniazid in rat plasma , brain, liver, and kidney, J Chromat Separation Techniq, 2012. 3(3):1-5. ISSN2157-7064 JCGST.

7. Kumar AKH, V Sudha, G Ramachandran. Simple and rapid method for simultaneous determination of isoniazid and acetylisoniazid in urine by hplc. Asian J Biomed and Pharm Sci. 2014. 4(34):46-50.

8. Kemenkes. Farmakope Indonesia V. Direktorat Jendral Bina Farmasi dan Alat Kesehatan. Kementerian Kesehatan RI. 2014. ISBN 987-602-235-463-5:567-9.

9. EMEA (European Medicines Agency). 2012. Guideline on bioanalytical method validation. EMEA/CHMP/EWP/192217/2009.

10. FDA (Food Drug Administration). Guidance for industry bioanalytical method of validation; available from: www.fda.gov/cder/guidance/index.htm. 2018.

11. Atta NF, A Galal, RA Ahmed. Voltammetric behavior and determination of isoniazid using PEDOT electrod in presence of surface active agents. Int J Electrochem Sci. 2011. 6(10):5097-113.

12. Oommen V, Ganesh G, Vadivel K, Kanthakumar P. The Henderson-Hasselbalch equation : a three dimensional teaching model.Indian Journal of Physiology and Pharmacology. 2016. 60(1):70-75.

13. Brown SA, NA Ezejiofor, OE Orisakwe. Pharmacokinetics of isoniazid with or without ofloxacin. International Current Pharmaceutical Journal. 2012.1(12):403-9.

14. Skoog DA, DM West, FJ Holler. SR Crouch. Fundamental of analytical chemistry. Ninth Edition. Mary Finch Publisher. 2014. ISBN-13: 978-0-495-55828-6. ISBN-10: 0-495-55828-1

15. Snyder LR, JJ Kirkland, JW Dolan. Introduction to modern Liquid Chromatography. Third Edit. United States of America: Wiley Publication. 2010.
Published
2019-10-29
How to Cite
YANTIH, Novi et al. Separation Selectivity of Isoniazid and Asetilisoniazid in Human Plasma In-vitro by High Performance Chromatography. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 2, p. 238-245, oct. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/717>. Date accessed: 26 dec. 2024. doi: https://doi.org/10.35814/jifi.v17i2.717.
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Vol 17 No 2 (2019): JIFI
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