The Lipid Profile and Aorta Histopathology on Hyperlipidemic Rat by Giving Green Coffee Extract

  • Fransiska Maria Christianty Faculty of Pharmacy, University of Jember
  • Diana Holidah Fakultas Farmasi, Universitas Jember
  • Fifteen Aprila Fajrin Fakultas Farmasi, Universitas Jember
  • Monica Cinuradha Aura Salsabina Fakultas Farmasi, Universitas Jember
  • Andrean Roni Fakultas Farmasi, Universitas Jember

Abstract

The high prevalence and risk of death from cardiovascular disease has led to the development of alternative treatments derived from nature. Green coffee has not undergone a roasting process. The product is claimed as a weight loser, even though the cardiovascular effects are still controversial. This study aim was to determine the impact of green coffee bean extract on rat lipid profiles and aortic histopathology. Green coffee bean extract made by a re-maceration method using 96% ethanol. Rats were induced with high-fat diet then treated with green coffee bean extract at a dose of 200; 400; and 800 mg/kg BB. Rats were sacrificed, blood was taken for lipid profile measurements and observations were made on histopathological preparations of rat aorta. The results showed that administration of green coffee bean extract (especially doses of 200 mg/kg BW) for 14 days in hyperlipidemic mice could significantly improve lipid profiles, except HDL levels. Microscopic observations showed that green coffee bean extract could enhance the rat aorta histopathology even though it did not match normal conditions. We can conclude that green coffee bean extract has potential effects to be developed as an alternative to traditional medicine that has antihyperlipidemic effects and cardiovascular protective effects.

References

1. World Health Organization (WHO). 2011. Global Atlas on Cardiovascular Disease Prevention and Control. Edited by: Mendis, S., Puska P., and Norrving, B. Geneva: WHO in Collaboration with World Heart Federation and World Stoke Organization.

2. World Health Organization (WHO). 2013. Cardiovascular Diseases (CVDs). Report on the Fact sheet No. 317. Geneva: WHO.

3. Kemenkes RI. 2014. Info Datin: Situasi Kesehatan Jantung. Jakarta: Pusat Data dan Informasi Kemenkes RI.

4. Kemenkes RI. 2013. Profil Kesehatan Indonesia 2012. Jakarta: Pusat Data dan Informasi Kemenkes RI.

5. Munzel T, Gori T, Bruno RM, and Taddei S. Translational medicine: is oxidative stress a therapeutic target in cardiovascular disease? Eur Heart J. 2010. 31:2741–9.

6. Favero G, Paganelli C, Buffoli B, Rodella L, and Rezzani R. Endothelium and Its Alterations in Cardiovascular Diseases: Life Style Intervention. BioMed Res Int. 2014. 2014: 1-28.

7. Nirosha K, Divya M, Vamsi S, and Sadiq M. A review on hyperlipidemia. Int. J Novel Trends Pharm Sci. 2014. 4(5):81-92.

8. Yu XH, Fu YC, Zhang DW, Yin K, and Tang CK. Foam cells in atherosclerosis. Clin Chim Acta. 2013. 424:245–52.

9. Wang H and Huo Y. Adhesion molecule and atherosclerosis – Part II: Pro Inflamatory Factor. In: George SJ and Johnson J, editors. Atherosclerosis: Molecullar and Cellular Mechanisms. British: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim; 2010. p. 43-62.

10. Gumulya D and Helmi IS. Kajian Budaya Minum Kopi Indonesia. Dimensi. 2017. 13(2): 153-72.

11. Dirjen Perkebunan. Statistik Perkebunan Indonesia 2015-2017: Kopi. Jakarta: Sekretariat Direktorat Jenderal Perkebunan, Direktorat Jenderal Perkebunan, Kementerian Pertanian; 2016. p. 48.

12. Onakpoya I, Terry R, and Ernst E. The use of green coffee extract as a weight loss supplement: a systematic review and meta-analysis of randomised clinical trials. Gastroenterol Res Pract. 2011. 2011:1-6.

13. Samadi M, Mohammadshahi M, and Haidari F. Green coffee bean extract as a weight loss supplement. J Nutr Disorders Ther. 2015. 5(4): 1-3.

14. Natania O and Musyabiq S. Efektivitas asam klorogenik dalam ekstrak kopi hijau untuk penurunan berat badan pasien obesitas. Majority. 2017. 7(1):94-99.

15. Buchanan R and Becket RD. Green coffee for pharmacological weight loss. Evid-Based Complementary Altern Med. 2013. 18(4):309-313.

16. Farah, A. Coffee: Emerging health effects and disease prevention, 1st ed. New York: WileyBlackwell Publising Ltd; 2012.

17. Ma Y, Gao M, and Liu D. Chlorogenic acid improves high fat diet-induced hepatic steatosis and insulin resistance in mice. Pharm Res. 2015. 32(4):1200-9

18. Cho AS, Jeon AM, Kim MJ, Yeo J, Seo KI, Choi MS, et al. Chlorogenic acid exhibits anti-obesity property and improves lipid metabolism in high-fat diet-induced-obese mice. Food Chem Toxicol. 2010. 48:937–43.

19. Setyono J, Nugroho DA, Mustofa, and Saryono. Efek orlistat, ekstrak biji kopi hijau, dan kombinasinya terhadap kadar adiponektin dan profil lipid. Jurnal Ners. 2014. 9(1):26–34.

20. Cappelletti S, Daria P, Sani G, and Aromatario M. Caffeine: cognitive and physical performance enhancer or psychoactive drug? Curr Neuropharmacol. 2015. 13:71-88.

21. Patay EB, Bencsik T, and Papp N. Phytochemical overview and medicinal importance of Coffea species from the past until now. Asian Pac J Trop Med. 2016. 9(12):1127–1135.

22. Aziz Z, Cyriac S, Beena V, and Philomina PT. Comparison of cholesterol content in chicken, duck and quail eggs. J Vet Anim Sci. 2012. 43: 64-66.

23. Nurfadilah, Nurainiwati SA, Agustini SM. Pengaruh pemberian minyak deep frying terhadap perubahan histopatologi jantung tikus putih (Rattus novergicus strain Wistar). Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. 2013. 9(1): 1-5.

24. Hasimun P, Sukandar EY, Adnyana IK, and Tjahjono DH. A simple method for screening antihyperlipidemic agents. Int J Pharm. 2011. 7(1):74-78.

25. Choi BK, Park SB, Lee DR, Lee HJ, Jin YY, Yang SH, et al. Green coffee bean extract improves obesity by decreasing body fat in high-fat-diet induced obese mice. Asian Pac J Trop Med. 2016. 2016:1-9.

26. Shimoda H, Seki E, and Aitani M. Inhibitory effect of green coffe bean extract on fat accumulation and body weight gain in mice. BMC Complem Altern M. 2006. 6(9):1-9.

27. Murase T, Misawa K, Minegishi Y, Aoki M, Ominami H, Suzuki Y, et al. Coffee polyphenols suppress diet-induced body fat accumulation by downregulating SREBP-1c and related molecules in C57BL/dJ mice. Am J Physiol Endocrinol Metab. 2011. 300: E122-33.

28. Nagla MF and Amr AR. Effect of green and roasted coffee beans extracts on some physiological parameters and histological structures in rats. Afr J Biol Sci. 2009. 5(2):181-95.
Published
2020-04-24
How to Cite
CHRISTIANTY, Fransiska Maria et al. The Lipid Profile and Aorta Histopathology on Hyperlipidemic Rat by Giving Green Coffee Extract. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 1, p. 21-27, apr. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/718>. Date accessed: 31 may 2020. doi: https://doi.org/10.35814/jifi.v18i1.718.
Section
Articles