In-Vitro Characterization of Nanoparticles and Antiagregation Tests on Brown Seaweed Extracts (Sargassum polycystum) Types of Sellulase Enzyme Hydrolysis

  • Kartiningsih Kartiningsih
  • Syamsudin Abdillah Fakultas Farmasi Universitas Pancasila
  • Partomuan Simanjuntak Fakultas Farmasi Universitas Pancasila
  • Cyntia Cyntia Fakultas Farmasi Universitas Pancasila
  • Haryo Haryo Fakultas Farmasi Universitas Pancasila
DOI: https://doi.org/10.35814/jifi.v17i2.720

Abstract

Brown seaweed contains fucoidan, a large molecular weight sulfate polysaccharide (about 100,000 Da) which has platelet antiagregation activity. This activity is achieved if the fucoidan has a small molecular weight (3900-7600 Da) so this activity can increase by hydrolized with sellulase Enzym. The purpose of this study was to obtain extract nanoparticles that meet physical quality requirements and have a higher platelet antiagregation activity than brown seaweed extract both before and after hydrolysis. Extraction was using kinetic maseration method using 80% ethanol after that using 2% calcium chloride solution. The results were dried and hydrolyzed with cellulase enzyme and nanoparticles were made by ionic gelation method. Nanoparticle characterization results in particle size of 552.8, polydispersity index of 0.569, potential zeta of +53.5 mV, and spherical shape. In-vitro testing results for platelet antiagregation activity showed the percentage of platelet aggregation inhibition of brown seaweed extract with a concentration 500µg / mL is 22.19% and extracts after hydrolysis was 57.94% and nanoparticles extract after hydrolysis was 72.93%. Extract nanoparticles meet physical quality requirements and extracted nanoparticles after hydrolysis have the highest platelet antiagregation activity compared to brown seaweed extract both before and after hydrolysis.

References

1. Septiana AT, Asnani A. Kajian sifat fisikokimia ekstrak rumput laut coklat Sargassum duplicatum menggunakan berbagai pelarut dan metode ekstraksi. Agrointek. 2016 Nov 9;6(1):22-8.

2. Sahri A. Mengenal potensi rumput laut: kajian pemanfaatan sumber daya rumput laut dari aspek industri dan kesehatan. Majalah Ilmiah Sultan Agung. 2018 Jun 2;44(118):95-116.

3. Pakidi CS, Suwoyo HS. Potensi Dan Pmanfaatan Bahan Aktif Alga Cokelat Sargassum sp. Octopus: Jurnal Ilmu Perikanan. 2017 Jun 30;6(1):551562.

4. Dewanto SM, Iskandar Y, Barliana MI. Potensi Rumput Laut Coklat Jenis Sargassum duplicatum yang berasal dari Perairan Menganti-Kebumen sebagai Antiagregasi Platelet. Fakultas Farmasi Universitas Padjajaran. 2011

5. Sinurat E, Marraskuranto E. Fucoidan from brown seaweed and its bioactivity. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology. 2013 May 8;7(3):131-8.

6. Palanisamy S, Vinosha M, Marudhupandi T, Rajasekar P, Prabhu NM. Isolation of fucoidan from Sargassum polycystum brown algae: Structural characterization, in vitro antioxidant and anticancer activity. International journal of biological macromolecules. 2017 Sep 1;102:405-12.

7. Thuy TT, Van TT, Hidekazu Y, Hiroshi U. Fucoidan from Vietnam Sargassum swartzii: Isolation, Characterization and Complexation with Bovine Serum Albumin. Asian Journal of Chemistry. 2012 Aug 1;24(8).

8. Chollet L, Saboural P, Chauvierre C, Villemin JN, Letourneur D, Chaubet F. Fucoidans in nanomedicine. Marine drugs. 2016 Jul 29;14(8):145.

9. Santoso, H. Preparasi dan Karakterisasi Nanopartikel Ekstrak Rumput Laut Coklat (Sargassum polycystum) Yang di Hidrolisis dengan Enzim Sellulase. 2018

10. Rawat M, Singh D, Saraf S, Saraf S. Nanocarriers: promising vehicle for bioactive drugs. Biological and Pharmaceutical Bulletin. 2006;29(9):1790-8.

11. Fitton JH. Therapies from fucoidan; multifunctional marine polymers. Marine drugs. 2011 Sep 30;9(10):1731-60.

12. Martien R, Adhyatmika A, Irianto ID, Farida V, Sari DP. Perkembangan teknologi nanopartikel sebagai sistem penghantaran obat. Majalah Farmaseutik. 2012;8(1):133-44.

13. Cosemans JM, Angelillo-Scherrer A, Mattheij NJ, Heemskerk JW. The effects of arterial flow on platelet activation, thrombus growth, and stabilization. Cardiovascular research. 2013 May 10;99(2):342-52.

14. Said KA. Penggunaan antiplatelet (aspirin) pada akut stroke iskemik. Co-ass Clinical at neurology departement dr. Zainoel Abidin Teaching Hospital, Faculty of Medicine University of Syiah Kuala. 2011;201.
Published
2019-10-29
How to Cite
KARTININGSIH, Kartiningsih et al. In-Vitro Characterization of Nanoparticles and Antiagregation Tests on Brown Seaweed Extracts (Sargassum polycystum) Types of Sellulase Enzyme Hydrolysis. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 2, p. 164-168, oct. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/720>. Date accessed: 18 apr. 2024. doi: https://doi.org/10.35814/jifi.v17i2.720.
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Vol 17 No 2 (2019): JIFI
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