Isolation and Identification of Phenolic Compounds from Macaranga hispida Blume Mull.Arg Leaves

  • Megawati Megawati Research Center for Chemistry, Indonesian Institute of Sciences(LIPI), Serpong
  • Sofa Fajriah Research Center for Chemistry, Indonesian Institute of Sciences(LIPI), Serpong
  • Galuh Widyawati Research Center for Chemistry, Indonesian Institute of Sciences(LIPI), Serpong
  • Akhmad Darmawan Research Center for Chemistry, Indonesian Institute of Sciences(LIPI), Serpong
DOI: https://doi.org/10.35814/jifi.v18i2.722

Abstract

Continuation of research on Macaranga plant has been conducted. In this study, the total
phenol content (TPC) of Macaranga hispida Blume Mull. Arg leaves extracts were investigated. Gallic
acid (1) and methyl gallate (2) have been isolated from the ethyl acetate fraction which highest phenolic
content (8.41 mg GAE/g). Serial techniques of chromatographic and spectroscopic methods, namely
extraction, fractionation, purifi cation, FTIR, LCMS, and FT-NMR were used to isolate and identify the
isolates. Cytotoxicity of compound 1 and 2 against P-388 cell line showed that compound 2 has higher
anticancer activity compared to compound 1 with IC50 value of 75.53 μg/mL.

References

1. Blattner FR, Weising K, Banfer G. Maschwitz U
and Fiala B. Molecular analysis of phylogenetic
relationships among myrmecophytic macaranga
species (Euphorbiaceae). Mol. Phyl. Evol. 2001;19:
331-44.

2. Magadula J Joseph. Phytochemistry and pharmacology
of the genus Macaranga: a review. Journal of Medical
Plant Research. 2014; 8(12):489-503.

3. Darmawan A, Suwarso WP, Kosela Sand Kardono
LBS. Macarangin a geranylated flavonoid and
anticancer active compound isolated from ethyl acetat
fraction of Macaranga gigantifolia. Ind. J. of Pharm.
2015; 26, 52–6.

4. Primahana G and Darmawan A. A fl avonoid glycoside
compound isolated from Macaranga gigantifolia Merr
leaves. J. Pure App. Chem. Res. 2017;6(1): 22-6.

5. Fajriah S, Megawati, and Darmawan A. Apigenin,
an anticancer compound isolated from Macaranga
gigantifolia Leaves. Journal Of Tropical Life Science.
2016;6(1):7-9.

6. Megawati, Saepudin E, Hanafi M, Darmawan A and
Lotulung PDN. Identifi cation and bioactivity studies
of fl avonoid compounds from Macaranga hispida
(Blume) Mull.Arg. Makara J. Sci. 2015;19: 96–100.

7. Megawati, Hanafi M, Saepudin E and Fajriah S. Isolasi
identifi cation and cytotoxic activity of scopolotin from
leaf of Macaranga hispida (Blume)Mull.Arg. J.Ilmu
Farmasian Indonesia. 2016;14 (1): 38-42.

8. Cai J, Zhao X-L, Liu A-W, Nian H, Zhang SH.
Apigenin inhibits hepatoma cell growththrough
alteration of gene expression patterns. Phytomedicine.
2011;18:366-73.

9. Babu BS, Stefano DA, Mohan BG, Pramod K,
Gabbriella I. New fl avonoid glycosidesfrom Aconitum
naviculare (Bruhl) Stapf, a medical herb from the
trans-Himalayan region of Nepal, J.Carbohydrat. Res.
2006; 341:2161-5.

10. Ding V, Zhang H-P, Duc Nm, Tuu NV, Qin G W. A
new geranylvfl avanone from Macaranga triloba. J.
Asian Nat. Prod. Res. 2006;8(2):155-158.

11. Ma J, Li Q, Zhao J, Ying G, Su Q, Ji Z. Eff ects of
apigenin on cell proliperation of human pancreatic
carcinoma cell line BxPC-3 invitro. J. Nanjing Med.
2007;21(2):94-8.

12. Nayaka HB, Ramesh LL, Madire KU, AshaT.
Antibacterial attributes of Apigenin isolated from
Portulacaoleracea L. Int. J. Bacteriol. 2014:1-8. https://
doi.org/10.1155/2014/175851

13. Kaur C and Kapoor HC. Anti-oxidant activity and total
phenolic content of some Asian vegetables. International
Journal of Food Science & Technology. 2002;37:153-
161. doi:10.1046/j.1365-2621.2002.00552.

14. Darmawan A, Fajriah S, Megawati, Dewijanti
ID, Banjarnahor S, Yuliani T, Hartati S, Mozef T,
Eff endi R and Swandiny G. Antioxidant activities
of phenolic compound isolated from the leaves
of Macaranga Allorobinsonii Whitemore, AIP
Conference Proceedings. 2016;1803: 020020
Published
2020-10-28
How to Cite
MEGAWATI, Megawati et al. Isolation and Identification of Phenolic Compounds from Macaranga hispida Blume Mull.Arg Leaves. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 2, p. 198-201, oct. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/722>. Date accessed: 31 dec. 2024. doi: https://doi.org/10.35814/jifi.v18i2.722.
Citation Formats
Issue
Vol 18 No 2 (2020): JIFI
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.