Soil Fungi Isolation from Situbondo District and Screening of Antibacterial Activities Against Pseudomonas Aeruginosa

  • Fawwas Batio Putra Pamungkas Universitas Jember
  • Bawon Triatmoko Universitas Jember
  • Ari Satia Nugraha Universitas Jember

Abstract

Bacterial resistance to antibiotics is a serious concern. The search for new antibacterial compounds
from medicinal plants can result in overexploitation of natural ingredients. Therefore, it is necessary to search for new alternative sources. This study was aimed to fi nd new antibacterial agents derived from soil fungi isolated from swampy soil in the Pasir Putih Beach area of Situbondo Regency in inhibiting Pseudomonas aeruginosa (P. aeruginosa). Isolation was carried out using Potato Dextrose Agar (PDA) dissolved in sea water. The isolation results showed that 5 isolates fungi. The fungi IS-STB-III-4 and IS-STB-III-5 have the potential to inhibit bacterial using antagonist tests. Potential soil fungi were fermented for 14 days and extracted with ethyl acetate as a solvent. The extraction results were dissolved in 10% DMSO with a concentration series of 1000 μg /mL, 2000 μg/mL, 4000 μg/mL, 6000 μg/mL, 8000 μg/mL which will be used for antibacterial activity test using the disc diff usion method. The extract activity test showed results that were directly proportional to the extract concentration. The analysis and profi ling of the extract using High Performance Liquid Chromatography (HPLC) instrument showed that the extract contained alkaloids which were then isolated and identifi ed by HPLC.

References

1. WHO. Who statistical profi le : World Health Organization on Behalf of the Special Programme for Research
and Training in Tropical Diseases. 2015. diambil dari http://who.int/gho/mortality_burden_disease/en/.
diakses 21 April, 2019

2. Weber JT. Antimicrobial Resistance Edisi 6. Switzerland: S. Karger AG; 2010. P. 35–50

3. Lestari ESJA, Severin PMG, Filius K, Kuntaman DO, Duerink U, Hadi H, Wahjono et.al. Antimicrobial resistance
among commensal isolates of escherichia coli and staphylococcus aureus in the indonesian population
inside and outside hospitals. Eur J Clin Microbiol Infect Dis. 2007. 27(1):45-51.

4. Rukmono P, Zuraida R. Uji kepekaan antibiotik terhadap pseudomonas aeroginosa penyebab sepsis
neonatorum. Sari Pediatri. 2013. 14(5) :332–6.

5. Zhu F, Qin C, Tao L, Liu X, Shi Z, Ma X, Jia J, et.al. Clustered patterns of species origins of nature-derived
drugs and clues for future bioprospecting. PNAS. 2011. 108(31) :12943–8.

6. Lestari ND. Skrining fi tokimia dan uji aktivitas antibakteri tumbuhan epifi t liken Parmelia cetrata Ach.
[skripsi]. Jurusan Farmasi Universitas Jember; 2018. hal 1-4.

7. Cannon P, Aguirre-Hudson B, Aime MC, Ainsworth AM, Bidartondo M, Gaya E, et al. Defi nition and diversity.
In: Willis KJ, editor. State of the world’s fungi. London: Royal Botanic Gardens – Kew; 2018. p. 1-12.

8. Gita T. Isolasi dan karakterisasi senyawa alkaloid ekstrak metanol sponge Clathria sp [skripsi]. Jurusan
Kimia Universitas Lampung; 2018. hal. 1-3.

9. Nugraha AS, Haritakun R, Lambert JM, Dillon CT, Keller PA. Alkaloids from the root of Indonesian Annona
muricata L. Nat Prod Res. 2021. 35(3): 481-9.

10. Pranata I. Pengaruh suhu dan waktu penyimpanan terhadap stabilitas triprolidin hcl dan pseudoephedrin
hcl dalam sediaan sirup obat fl u [tugas akhir]. Jurusan Analis Kimia Politeknik Negeri Bandung; 2012. hal
9 – 26.
Published
2021-04-30
How to Cite
PAMUNGKAS, Fawwas Batio Putra; TRIATMOKO, Bawon; NUGRAHA, Ari Satia. Soil Fungi Isolation from Situbondo District and Screening of Antibacterial Activities Against Pseudomonas Aeruginosa. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 19, n. 1, p. 73-79, apr. 2021. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/750>. Date accessed: 20 june 2021. doi: https://doi.org/10.35814/jifi.v19i1.750.
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Articles