Formulation and Inhibitor Test of Peel-Off Mask Thyrosinase Extract 96% Ethanol Extract Lime Peel (Citrus auratifolia)

  • Ari Widayanti Universitas Muhammadiyah Prof Dr. HAMKA
  • Rini Prastiwi Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA
  • Kartika Tiara Wijayanti Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA

Abstract

Tyrosinase is an enzyme that is widely distributed in microorganisms, plants and animals. Tyrosinase
is the main enzyme involved in the biosynthesis of melanin. Melanin has a very important role in skin protection, especially against exposure to ultraviolet rays which are harmful to the skin. This study aims to test the tyrosinase inhibitor in the peel-off mask of lime peel extract (Citrus aurantifolia). Lime peel extract is formulated into a peel-off mask with extract content of 0% (F1), 15% (F2) and 25% (F3) respectively and with the gelling agent PVA (Polyvinyl alcohol). The physical stability test was carried out by measuring the pH, the results obtained were F2 pH 6.85 and F3 pH 6.78. The analysis of tyrosinase inhibitor activity was carried out using a UV-Vis spectrophotometer and seen from the percent inhibition value obtained. The percent inhibition results obtained from the peel-off mask of lime extract were 17.89% (F2) and 18.86% (F3). Lime peel has the potential as a tyrosinase inhibitor.

References

1. Okwu. Citrus fruits: A rich source of phytochemicals and their roles inhuman health. Int J Chem Sci. 2008.6(2): 451-471

2. Abrahimi, A. In vitro antioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh
juice from Citrus hystrix and C.maxima fruits. School of Life Sciences. Department of Environmental Sciences.
Bharathiar University. Coimbatore. 2014 641 046. Tamil Nadu. India

3. Sasaki. K dan Yoshizaki F.Nobiletin as a tyrosinase inhibitor from the peel of citrus fruit. Biol Pharm Bull.
2002. 25: 806-808

4. Costin, G. E., and Hearing, V. J. Human Skin Pigmentation: Melanocytes Modulated Skin Color in Response
to Stress. FASEB Journal. 2007. 21(4):976-94.

5. Khasanah. I. Uji Aktivitass Antioksidan Ekstrak Etanolik Kulit buah Jeruk Nipis (Citrus aurantifolia) Dengan
Metode DPPH (1,1-difenil-2-pikrilhidrazil). Semarang: Fakultas Farmasi Universitas Wahid Hasyim Semarang.
2014.

6. Hindun. S. Potensi Limbah Kulit Jeruk Nipis (Citrus aurantifolia) Sebagai Inhibitor Tirosinase. Jawa Barat:
Fakultas Farmasi Universitas Padjadjaran Jawa Barat. 2017

7. Reny, S.S., D. Mulyati, A. Gadri. Formulasi sediaan masker gel peel off ekstrak daun pepaya (Carica Papaya
L) sebagai antijerawat dan uji aktivitasnya terhadap bakteri Propionibacterium acne. Prosding Penelitian
SpeSIA Unisba.2015. 662 – 670

8. Departemen Kesehatan RI. Farmakope Herbal Indonesia. Edisi 1. Jakarta. 2008. Hlm: 169-175

9. Eliyanoor Basyar. Penuntun Praktikum Farmakognosi Mikroskopik dan Makroskopik. Jakarta. Binu Ilmu
Mandiri. 2012

10. Departemen Kesehatan RI. Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jendral Pengawasan Obat Dan Makanan Direktorat Pengawasan Obat Tradisional, Jakarta. 2000. Hlm. 31-32

11. Sudarmadji, S.; Haryono B.; Suhardi. Prosedur Analisis untuk Bahan Makanan dan Pertanian. Yogyakarta.
Liberty. 2003.

12. Martin, A., J. Swarbrick, and A Cammarata. Farmasi Fisik: Dasar-dasar Farmasi Fisik dalam Ilmu Farmasetik.
Edisi Ketiga. Penerjemah: Yoshita. Jakarta: UI Press. 1993. Hal. 1124-1187.

13. Ardana M Vebry A, dan Arsyik I. Formulasi dan Optimasi gel HMPC (Hidroxy Prophyl Methyl Cellulose)
dengan Berbagai Variasi Konsentrasi. Kalimantan Timur. Dalam: Jurnal Trop Pharm Chem. 2015. Vol.3
No. 2 p-ISSN 2087-7099, e-ISSN 2407-6090. Hlm.4-11

14. Rasyad AA, Frenny Z, dan Ni Wayan LS. Formulasi dan Uji Aktivitas Anti Bakteri Masker Peel-off Serbuk
Getah Pepaya Muda dan Madu Hitam. 2016.

15. Departemen Kesehatan RI. Farmakope Indonesia Edisi V. Jakarta: Direktorat Jenderal Pengawasan Obat dan
Makanan. 2014. Hlm. 24, 1071

16. Arung, E. T., K. Shimizu., and R. Kondo. Inhibitory Effect of Artocarpanone from Artocarpus heterophyllus
on Melanin Biosynthesis. J. Biol. Pharm. Bull. 2006.

17. Chang TS. Tyrosinase and Tyrosinase Inhibitor. Journal of Biocatalysis and Biotransformation. 2012. 1 (2): 1-2

18. Hasemi SM, Emami S. Kojic Acid-derived Tyrosinase Inhibitors: Synthesis and Bioactivity. Pharmaceutical
and Biomedical Research. 2015.1(1): 1-17
Published
2021-04-30
How to Cite
WIDAYANTI, Ari; PRASTIWI, Rini; WIJAYANTI, Kartika Tiara. Formulation and Inhibitor Test of Peel-Off Mask Thyrosinase Extract 96% Ethanol Extract Lime Peel (Citrus auratifolia). JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 19, n. 1, p. 80-86, apr. 2021. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/764>. Date accessed: 28 sep. 2021. doi: https://doi.org/10.35814/jifi.v19i1.764.
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Articles