Phytochemical Investigation and Antibacterial Activity Ethanol Extract of Papaya Seeds (Carica papaya L.) Applicated for Gel Product

  • Wahyuning Setyani Fakultas Farmasi Universitas Sanata Dharma
  • Jacinda Yakub Fakultas Farmasi Universitas Sanata Dharma
  • Octavianus Yandri Sanata Dharma University
  • Viola Resti Kawan Fakultas Farmasi Universitas Sanata Dharma
  • Theresia Jenny Haryanto Fakultas Farmasi Universitas Sanata Dharma
  • I Made Myasa Darmika Fakultas Farmasi Universitas Sanata Dharma
DOI: https://doi.org/10.35814/jifi.v18i1.774

Abstract

Papaya seeds has many benefits in medicine. One of the benefits that have been studied is it’s antibacterial activity against Staphylococcus aureus. This study aims to determine the phytochemical compounds of papaya seed ethanol extract and their antibacterial activity against Staphylococcus aureus bacteria. The ethanol extract of papaya seeds was obtained through maceration method using 95% ethanol as a solvent. Maceration was carried out by weighing 85 grams of simplicia powder, macerated using 500 mL of 95% ethanol then left for 4 days while stirring occasionally, then filtered to produce filtrate and residue. The remaining residue is then soaked again (remaserated) with 250 ml of 95% ethanol and left for 2 days. After 2 days, the sample is filtered to produce filtrate and residue. All filtrates were evaporated using a rotary evaporator to obtain a thick extract. Phytochemical screening results showed the presence of flavonoids, alkaloids, tannins, saponins, and phenolic compounds in ethanol extracts of papaya seeds. The antibacterial activity test of papaya seed ethanol extracts towards Staphylococcus aureus was carried out through in vitro by disc diffusion method with several concentrations. Antibacterial test showed that concentrations of 2%, 4%, and 6% of papaya seeds ethanol extracts was moderate antibacterial activity and at concentrations of 8% and 10% the antibacterial activity was classified as strong against Staphylococcus aureus.

References

Davis, W.W., Stout, T.R., 1971. Disc Plate Method of Microbiological Antibiotic Assay. II. Novel Procedure Offering Improved Accuracy. Applied microbiology, 22(4), pp 666–70.

2. Eke, O.N., Augustine, A.U., Ibrahim, H.F., 2014. Qualitative Analysis of Phytochemicals and Antibacterial Screening of Extracts of Carica papaya Fruits and Seeds. International Journal of Modern Chemistry, 6(1), pp 48–56.

3. Ikalinus, R., Widyastuti, S., Luh, N., Setiasih, E., 2015. Skrining Fitokimia Ekstrak Etanol Kulit Batang Kelor (Moringa oleifera). Indonesia Medicus Veterinus, 4(1), pp 71–79.

4. Kharisma, Y., 2017. Tinjauan Pemanfaatan Tanaman Pepaya dalam Kesehatan. Universitas Islam Bandung, 1–14.

5. Lohidas, J., Manjusha, S., Jothi, G.G.G., 2015. Antimicrobial Activities of Carica papaya L . Plant Archives, 15(2), pp 1179–1186.

6. Muharni, Fitrya, Farida, S., 2017. Uji Aktivitas Antibakteri Ekstrak Etanol Tanaman Obat Suku Musi di Kabupaten Musi Banyuasin, Sumatera Selatan. Jurnal Kefarmasian Indonesia, 7(2), pp 127–135.

7. Patel, N., Meshram, D.B., Limited, G.B., Patel, P., Dhara, P., 2014. Phytochemical Analysis and Antibacterial
Activity of Moringa oleifera. International Journal of Medicine and Pharmaceutical Sciences (IJMPS), 4(2), pp 27–34.

8. Setiaji, A., 2009. Efektifitas Ekstrak Daun Pepaya Carica papaya L. untuk Pencegahan dan Pengobatan Ikan Lele Dumbo Clarias sp yang Diinfeksi Bakteri Aeromonas hydrophilia. Institut Pertaninan Bogor.

9. Setyani, W., Setyowati, H., and Ayuningtyas, D., 2016. Pemanfaatan Ekstrak Terstandardisasi Daun Som Jawa (Talinum paniculatum (Jacq.) Gaertn) Dalam Sediaan Krim Antibakteri Staphylococcus aureus. Jurnal Farmasi Sains dan Komunitas, 13 (1), 48 – 49

10. Torar, G.M.J., Lolo, W.A., Citraningtyas, G., 2017. Uji Aktivitas Antibakteri Ekstrak Etanol Biji Pepaya (Carica papaya L .) terhadap Bakteri Pseudomonas Aeruginosa dan Staphylococcus aureus. Pharmacon Jurnal Ilmiah Farmasi, 6(2), pp 14–22.
Published
2020-04-28
How to Cite
SETYANI, Wahyuning et al. Phytochemical Investigation and Antibacterial Activity Ethanol Extract of Papaya Seeds (Carica papaya L.) Applicated for Gel Product. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 1, p. 96-100, apr. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/774>. Date accessed: 27 apr. 2024. doi: https://doi.org/10.35814/jifi.v18i1.774.
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Vol 18 No 1 (2020): JIFI
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