In-Vitro Antihyaluronidase Activity and Physical Properties of Alpinia zerumbet Rhizome Extract-Loaded Nanostructured Lipid Carrier
The rhizome of Alpinia zerumbet (Pers.) B.L. Burtt & R.M. Sm) contains phenolic compounds, has antihyaluronidase activity which can reduce wrinkles on the skin. This study aims to determine the physical characteristics and antihyaluronidase activity of the nanostructured lipid carrier (NLC) of the Alpinia zerumbet rhizome extract in-vitro. Alpinia zerumbet rhizome powder (4/18) was extracted by kinetic maceration method using 70% ethanol solvent, concentrated with a rotary vacuum evaporator at 100 mm Hg at a temperature of ± 50oC. The resulting extract was prepared into NLC nanoparticles using polyethyleneglycol 8-beeswax, isopropyl myristate and acrylyl glucoside with the high speed homogenization-ultrasonication method. The resulting NLC extracts were characterized by nanoparticle size and morphology, polydispersity index and zeta potential and antihyaluronidase activity test was determined using the Sigma Aldrich and Tu & Tawata methods with slight modifications. The results of the NLC characterization of Alpinia zerumbet rhizome extract were 171.3 nm; 0.37; -26.8 mV; and spherical shape. The results of the antihyaluronidase activity of Alpinia zerumbet rhizome extract loaded NLC was 552.75 µg/mL. It was concluded that the Alpinia zerumbet rhizome extract loaded NLC can be formulated into topical preparations which have the potential to be antihyaluronidase with the ability to reduce wrinkles on the skin.
2. Papakonstantinou E, Roth M, Karakiulakis G. Hyaluronic acid: A key molecule in skin aging. From Basel, Switzerland. Journal Dermato Endocrinology, 2012; 4:3, p 253-258.
3. Lee A, Grummer SE, Kriegel D, Marmur E. Hyaluronidase. Dermatologic Surg. 2010,36:1071–7.
4. Kumud, M., Sanju, N. In-vitro evaluation of antioxidant, anti-elastase, anti-collagenase, anti-hyaluronidase activities of Safranal and determination of its sun protection factor in skin photoaging. Bioorg Chem. 2017;77:159–67. doi:https://doi.org/10.1016/j.bioorg.2017.12.030
5. Elzaawely AA, Xuan TD, Tawata S. Changes in essential oil, kava pyrones and total phenolics of Alpinia zerumbet (Pers.) B.L. Burtt. & R.M. Sm. leaves exposed to copper sulphate. Environ Exp Bot. 2007; 59 (3):347–53.
6. Lim T. K. Alpinia zerumbet. Edible Medicinal and Non-Medicinal Plants. Springer International Publishing, 2016, p 196-213
7. Murakami S, Li W, Matsuura M, Satou T, et al. Composition and seasonal variation of essential oil in Alpinia zerumbet from Okinawa Island. Nat Med; 2009, p 204–208.
8. Chompoo J, Upadhyay A, Fukuta M, Tawata S. Effect of Alpinia zerumbet components on antioxidant and skin diseases-related enzymes. BMC Complement Altern Med: 2012; 12 (1) :106. p 1-9.
9. Azwanida, N. N. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Med Aromat Plants, 2015;4(3). doi:https://doi.org/10.4172/2167-0412.1000196
10. Lacerda, S. P., Cerize, N. N. P., Re, M. I. Preparation and characterization of carnauba wax nanostructured lipid carriers containing benzophenon-3. International Journal of Cosmetic Science, 2011; 33:112-21. doi:https://doi.org/10.1111/j.1468-2494.2010.00626
11. Lason, E., Sikora, E., Ogonowski, J. Influence of proses parameters on properties of NLC formulation. Acta Biochimica Polonica (ABP), 2013;60(4):773-7. doi:https://doi.org/10.18388/abp.2013_2056
12. Chaudary, H., Jadhav, K., Kadam, V. J. Formulation and evaluation of nanostructured lipid carriers containing glipizide. World Journal of Pharmacy & Pharmaceutical Sciences, 2016; 5(4):1424-37. doi:https://doi.org/10.20959/wjpps20164-6398
13. Tu PTB, Tawata S. Antioxidant, antiaging, and antimelanogenic properties of the essential oils from two varieties of Alpinia zerumbet. MDPI Journal. 2015; 20(9):16723-40
14. Joseph, E., & Singhvi, G. Multifunctional nanocrystals for cancer therapy: a potential nanocarrier. Nanomaterials for Drug Delivery and Therapy. 2019;91–116. doi:https://doi.org/10.1016/b978-0-12-816505-8.00007-2.
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