Formulation of NLC Gel from Callus Mulberry Leaf Extract Induced by NAA and BAP

  • Faizatun Faizatun Fakultas Farmasi Universitas Pancasila
  • Erlindha Gangga Universitas Pancasila
  • Sarah Anindita Universitas Pancasila
  • Titiek Martati Universitas Pancasila
  • Nur Miftahurrohmah Universitas Pancasila
DOI: https://doi.org/10.35814/jifi.v18i1.814

Abstract

Mulberry Leaf (Morus alba L.) contains oxyresveratrol has the potential as a skin lightener. The activity of mulberry leaf callus from tissue culture is unknown as an inhibitor of the tyrosinase enzyme. The purpose of this study was to determine the activity callus extracts of mulberry leaf  which obtained from tissue culture with the addition of growth regulator NAA 1.0 ppm and BAP 2.5 ppm and to formulate callus extract in Nanostructured Lipid Carrier and its characteristics. Callus was extracted by maceration-sonication. NLC is made by solvent evaporation method, NLC characterization including particle size, polydispersity index, zeta potential and particle morphology. The best NLC was made into a gel and evaluated organoleptic, viscosity, flow properties, pH, and tyrosinase enzyme inhibitory on extracts and NLC gels. The characterization of NLC includes particle size of 189.8 nm-632.8 nm; polydispersity index, 0.387-0.582. The morphology of NLC were spherical and zeta potential of -7.37 mV. NLC gel was semi-solid, greenish yellow, homogeneous, viscosity 530000 cPs, plastic thixotropic flow properties, pH 5.26, and inhibitory activity of tyrosinase enzymes (IC50) of callus extract and NLC gel has 72.51 µg/ml  and 79.69 µg/ml, respectively. It can be concluded that callus extract of Mulberry leaf can be prepared into Nanostructured Lipid Carriers, NLC gels are physically and chemically stable and have the potential of lightening activity.

References

1. Hakim EH, Syah YM, Juliawati LD, Mujahidin D. Aktifitas antioksidan dan inhibitor tirosinase beberapa stilbenoid dari tumbuhan Moraceae dan Dipterocarpaceae yang potensial untuk bahan kosmetik. Bandung: Institut Teknologi Bandung; 2008. 13(2): h.33-40

2. Hendaryono DPS, Wijayani A. Teknik Kultur Jaringan; Pengenalan dan Petunjuk Perbanyakan Tanaman Secara Vegetatif-Modern. Yogyakarta: Kanisius. 1994. h.26-28, 31, 33,72

3. Santoso B, Prayudyaningsih R, Rismawati A. Kultur jaringan BAP dan NAA tanaman Murbei (Morus alba L.) varietas KI 14 secara in-vitro. Jurnal Penelitian Hutan dan Konservasi Alam. 2006;6(2): h.157-164

4. Faizatun, Anwar E., Djajadisasra J., Mardliyati E., The Study of Antioxidant and Antityrosinase Activity of Extract from Mulberry Root (Morus alba L.). J. Pharm. Sci. & Res. Vol. 9(10), 2017, 2004-2008.

5. Faizatun F. and Asto Dayu S., In Vitro Determination of Sun Protection Factors on Ethanol Extract and Nanostructured Lipid Carrier-Based Gel Extract of Mulberry Root (Morus Alba L.). Asian J Pharm Clin Res, Vol 11, Special issue 1, 2018. 138-140

6. Zhou, J., et al. Variations in the Levels of Mulberroside A, Oxyresveratrol, and Resveratrol in Mulberries in Different Seasons and during Growth, The ScientificWorld Journal, 2013. Article ID 380692.
Published
2020-04-28
How to Cite
FAIZATUN, Faizatun et al. Formulation of NLC Gel from Callus Mulberry Leaf Extract Induced by NAA and BAP. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 1, p. 123-129, apr. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/814>. Date accessed: 05 nov. 2024. doi: https://doi.org/10.35814/jifi.v18i1.814.
Citation Formats
Issue
Vol 18 No 1 (2020): JIFI
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.