Culture Broth Activity of Antibiotic Producer Bacteria (P301 Isolate) Against Staphylococcus aureus ATCC 25923 and Time Optimization of Secondary Metabolite Production

  • NANIK SULISTYANI Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta
  • IIN NARWANTI Fakultas Farmasi Universitas Ahmad Dahlan Yogyakarta

Abstrak

Isolate P301 is an antibiotic producer bacteria isolated from rice plant ryzospher. This study was intended to identify the antibacterial activity of its culture broth againts Staphylococcus aureus ATCC 25923 and to identify the time optimization profileof secondary metabolite production from isolate P301 as producer antibiotics. Antibacterial activity was carried out using cup plate method by measuring the inhibitory zone around the wells. Optimization profile of production time of secondary metabolite was determined by plotting of the inhibition zone diameter versus incubation time. The result showed that isolate P301 culture broth inhibited the growth of S. aureus. According to the activity assay againts S. aureus, the optimum time for antibiotic production is after doing incubation for at least 11 days.

Diterbitkan
2017-09-23
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SULISTYANI, NANIK; NARWANTI, IIN. Culture Broth Activity of Antibiotic Producer Bacteria (P301 Isolate) Against Staphylococcus aureus ATCC 25923 and Time Optimization of Secondary Metabolite Production. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 13, n. 2, p. 181-186, sep. 2017. ISSN 2614-6495. Tersedia pada: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/82>. Tanggal Akses: 09 aug. 2025
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Terbitan
Vol 13 No 2 (2015): JIFI
Bagian
Articles
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