Effect of Aspartame Oral Administration to AST and ALT Serum Levels and White Rat’s Histopatology Description Liver

  • Ros Sumarny Universitas Pancasila
  • Sondang Khairani Universitas Pancasila
  • Mohamad Rega Satria Ramadhan Universitas Pancasila
DOI: https://doi.org/10.35814/jifi.v18i1.830

Abstract

Aspartame is low calorie sweetener metabolized into aspartic acid, phenylalanine and methanol, methanol cause damage to the liver. The purpose this study to determine the effect rats of oral aspartame administration for 90 days on biochemical parameters AST, ALT and liver histopathology. Rats were divided randomly into four groups: normal control, dose groups 126, 252, 504 mg/kgBW. Blood samples were taken on the 0th, 30th, 60th and 90th days to measure AST, ALT levels and 90th day to see liver histopathology. AST and ALT measurements using UV-VIS spectrophotometry with a wavelength of 340 nm. The levels of AST and ALT on the 90th day in the normal group 41,55 and 48,40 UI/L. Dose groups 126, 252, and 504 mg / kgBW were low doses 54,62 and 57,72 UI/L, 66,88 and 70,47 UI/L, 77,50 and 80,03 UI/L. Rats histopathology liver of aspartame showed hemorrhage. Concluded aspartame can increase of AST and ALT levels in rats blood serum and liver damage.

References

1. D EPR, Yerizel E. Artikel Penelitian Pengaruh Pemberian Aspartam terhadap Kadar Glukosa Darah Tikus Diabetes Melitus Diinduksi Aloksan. 2014;3(3):370–5.

2. Hassan M, Hassan MI. Low Intake of Aspartame Induced Weight Gain and Damage of Brain & Liver Cells in Weanling Syrian Hamsters Low Intake of Aspartame Induced Weight Gain and Damage of Brain & Liver Cells in Weanling Syrian Hamsters. J Food Nutr Reaserch. 2016;4 (3)(April):152–6.

3. Agarwal A, Gutch M, Kumar S, Mohd SR, Kumar GA, Kumar KG. Nonnutritive sweeteners : Pros and cons. 2016;4–9.

4. Badan Pengawas Obat dan Makanan Republik Indonesia. Peraturan Kepala Badan Pegawas Obat dan Makanan Republik Indonesia Nomor 4 Tahun 2014 Tentang Batasan Maksimum Penggunaan Bahan Tambahan Pangan Pemanis. 2014;1–28.

5. Lebedev I, Park J, Yaylaian R. Popular Sweeteners and Their Health Effects. 2010;28–9.

6. Mourad M. Effect of aspartame on some oxidative stress parameters in liver and kidney of rats. 2011;5(June):678–82.

7. Avenue N. Oxidative Damage Caused by Air Formaldehyde Exposure in Organ of Mice. 2005;3813–6.

8. Prokić MD, Paunović MG, Matić MM, Đorđević NZ, Ognjanović BI, Štajn AŠ, et al. Effect of aspartame on biochemical and oxidative stress parameters in rat blood. Arch Biol Sci. 2015;67(2):535–45.

9. Alwaleedi SA. Alterations in antioxidant defense system in hepatic and renal tissues of rats following aspartame intake. J Appl Biol Biotechnol. 2016;4(2):46–52.

10. Finamor I, Pérez S, Bressan CA, Brenner CE, Rius-pérez S, Brittes PC, et al. Redox Biology Chronic aspartame intake causes changes in the trans-sulphuration pathway , glutathione depletion and liver damage in mice. Redox Biol [Internet]. 2017;11(January):701–7. Available from: http://dx.doi.org/10.1016/j.redox.2017.01.019

11. Dali S, Kusuma AT, Anar AW. Analisis Kandungan Aspartam yang Terdapat pada Minuman Jajanan Anak Sekolah yang Beredar di Makassar dengan Metode HPLC. 2013;5(2):162–8.

12. Adaramoye OA, Akanni OO. Effects of long-term administration of aspartame on biochemical indices , lipid profile and redox status of cellular system of male rats. 2016;27(1):29–37.

13. Silbernagl S, Lang F. Teks & Atlas Berwarna Patofisiologi. Penerbit Buku Kedokteran EGC. 2007
Published
2020-04-25
How to Cite
SUMARNY, Ros; KHAIRANI, Sondang; RAMADHAN, Mohamad Rega Satria. Effect of Aspartame Oral Administration to AST and ALT Serum Levels and White Rat’s Histopatology Description Liver. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 1, p. 53-60, apr. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/830>. Date accessed: 20 apr. 2024. doi: https://doi.org/10.35814/jifi.v18i1.830.
Citation Formats
Issue
Vol 18 No 1 (2020): JIFI
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.