Comparison of DNA Isolation Methods using FTA Card: Wizard® Kit Genomic DNA Purification, PureLink® Genomic DNA, and Chelex-100

  • Dyah Aryani Perwitasari Universitas Ahmad Dahlan
  • Imaniar Noor Faridah Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
  • Yeni Alfiana Ratnasari Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
  • Kania Agustina Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia
  • Ikrimah Nisa Utami RSUD Dr Moewardi, Solo
  • Rita Maliza Faculty of Science and Applied Technology, Universitas Ahmad Dahlan, Yogyakarta

Abstract

FTA Cards are an easy way to collect blood samples, deliveries, and stores. The total
volume of blood samples is less is needed when compared to the blood samples taken through a vein
(venipuncture). The purpose of this research is a method that can be applied to isolate DNA samples
from FTA Cards using the Wizard® Genomic DNA Purifi cation Kit (Promega), PureLink® Genomic
DNA Kits, and Chelex-100. This research was a type of experimental research, and data analysis was
showed in qualitative and quantitative descriptively. The samples used were 16 samples of human blood
taken using a lancet and then placed on the FTA Card, sampling was done by accidental sampling
technique. The variations of the PureLink® Genomic DNA kits method with the addition of SDS 10%
showed thin and smear DNA bands on agarose gels. Furthermore, the Chelex-100 method showed a
clear DNA band. The purity data obtained varied between 1 to 1.1 and concentrations between 190 to
950 ng/μl. The conclusion of this study is DNA kits method with the addition of 10% SDS and the
Chelex-100 method still have impurities, with the purity value of DNA was less than 1.8 and a low
concentration to be used as a DNA template for further molecular analysis.

Author Biographies

Imaniar Noor Faridah, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia

Faculty of Pharmacy, UAD

Yeni Alfiana Ratnasari, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia

Faculty of Pharmacy, UAD

Kania Agustina, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia

Faculty of Pharmacy, UAD

Ikrimah Nisa Utami, RSUD Dr Moewardi, Solo

RSUD Dr Moewardi, Soo

Rita Maliza, Faculty of Science and Applied Technology, Universitas Ahmad Dahlan, Yogyakarta

Faculty of Science and Applied Technology, UAD

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Published
2020-10-28
How to Cite
PERWITASARI, Dyah Aryani et al. Comparison of DNA Isolation Methods using FTA Card: Wizard® Kit Genomic DNA Purification, PureLink® Genomic DNA, and Chelex-100. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 2, p. 241-245, oct. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/838>. Date accessed: 02 dec. 2020. doi: https://doi.org/10.35814/jifi.v18i2.838.
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Articles