Isolation and Identification of Flavonoid Compounds in n-Butanol Phase Mulberry Leaves (Morus alba L.) using Spectrophotometry

  • RATNA DJAMIL UNIVERSITAS PANCASILA
  • FATIMAH BAKRIYYAH UNIVERSITAS PANCASILA

Abstrak

Mulberry (Morus alba L.), Moraceae, is one of plant in Indonesia that widely utilized in traditional medicine. Mulberry leaf contains many chemical compounds such as flavonoids, which shows a variety of biological activities and pharmacological properties. The purpose of this research is to investigate the flavonoid compounds contained in mulberry leaves. The n-butanol phase phytochemical screening showed the presence of compounds of flavonoids, saponins, tannins and coumarin. Isolation and identification of flavonoid compound from n-butanol phase in methanol extract of mulberry leaves has been done with paper chromatography method using n-butanol–acetic acid–water (4:1:5) eluent. The result of identification with visible- ultraviolet spectrophotometer suspected contains flavonoids, which NB III isolates is a flavonol compounds with OH group at 5, 4’ position, and o-diOH on A rings and prenil group at position 6. NB IV isolates are flavonol compounds with OH group at 5, 4’ position, and o-diOH on A rings and prenil group at position 6. NB V isolates are flavonol compounds with o-diOH on the A rings (6,7 or 7,8). NB VI isolates are dihydroflavonol with groups of o-diOH on A rings (6,7 or 7,8) and OH group at 4’position.

Diterbitkan
2017-09-23
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DJAMIL, RATNA; BAKRIYYAH, FATIMAH. Isolation and Identification of Flavonoid Compounds in n-Butanol Phase Mulberry Leaves (Morus alba L.) using Spectrophotometry. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 13, n. 2, p. 194-200, sep. 2017. ISSN 2614-6495. Tersedia pada: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/85>. Tanggal Akses: 12 july 2025
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