A Phytochemical and Antioxidant Evaluation of Piper hispidum Sw. Collected from Gunung Malang Village on Different Vegetation

-

  • Vivi Anggia UIN Syarif Hidayatullah Jakarta
  • Rindita Rindita Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA
  • Fauziah Ulfa Ramadhany Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA

Abstract

Jamaican pepper (Piper hispidum Sw.) is a wild plant, member of Piperaceae family and invasive plant species reported used to heal wounds and treat symptoms of skin leishmaniasis. This study aims to explore phytochemicals of Piper hispidum, especially phenolic content and antioxidant capacity, in two different abiotic condition of the forest, shaded and open area. Exploration of P. hispidum was conducted in Gunung Malang Village, Halimun Salak Mountain National Park (TNGHS). The sample was extracted with the ultrasonic method. The phenolic level and antioxidant activity were determined with Folin Ciocalteu and DPPH method where gallic acid was used as a standard. The total phenolic content assay showed 70% ethanol extract of P. hispidum Sw. leaves from the shaded and open forest were 17.4775 mg GAE/g and 12.6137 mg GAE/g of sample respectively. The antioxidant activity assay showed that IC50 of the sample from shaded and open forest were 94.8414 and 94.3412 ppm and gallic acid was 7.9817 ppm. This study showed that differences in vegetation and environmental abiotic factor may contributed in the amount of chemical compounds where open vegetation exposed to the sunlight had lower values than shaded but not significantly affected the antioxidant value.

References

1. Rindita, Anggia V, Rahmaesa E, Devi RK, Alawiyah LF. Exploration, phenolic content determination, and antioxidant activity of dominant pteridophytes in Gunung Malang Village, Mount Halimun Salak National Park, Indonesia. Biodiversitas. 2020. 21: 3676-3682.

2. Quijano-Abril MA, Callejas-Posada R, Miranda- Esquivel DR. Areas of endemism and distribution patterns for Neotropical Piper species (Piperaceae). Journal of Biogeography. 2006. 33: 1266–1278.

3. Michel JL, Chen Y, Zhang H, Huang Y, Krunic A, Orjala J, Veliz M, Soni KK, Soejarto DD, Caceres A et al. Estrogenic and serotonergic butenolides from the leaves of Piper hispidum Swingle (Piperaceae). J Ethnopharmacol. 2010. 129(2): 220–226.

4. Munnawaroh, E., Yuzammi. The diversity and conservation of piper (Piperaceae) in Bukit Barisan Selatan National Park, Lampung Province. Media Konservasi 2017. 22 (2): 118-128.

5. Salehi B, Zakaria ZA, Gyawali R, Ibrahim SA, Rajkovic J, Shinwari ZK et al. Piper species: A comprehensive review on their phytochemistry, biological activities and applications. Molecules. 2019. 24: 1364.

6. Santana AI, Vila R, Cañigueral S, Gupta MP. Chemical composition and biological activity of essential oils from different species of piper from Panama. Planta Med. 2016. 82: 986–991.

7. Navickiene HMD, AleÂcio AC, Kato MJ, Bolzani VDS. Antifungal amides from Piper hispidum and Piper tuberculatum. Phytochemistry. 2000. 55: 621- 626.

8. Jenett-Siems K, Mockenhaupt FP, Bienzle U, Gupta MP, Eich E. In vitro antiplasmodial activity of Central American medicinal plants. Trop Med Int Health. 1999. 4(9): 611-615.

9. Costa GM, Endo E. H., Cortez D.A.G., Nakamura T.U., Nakamura C.V., Dias Filho B.P. Antimicrobial effects of Piper hispidum extract, fractions and chalcones against Candida albicans and Staphylococcus aureus. Journal De Mycologie Médicale. 2016. 26(3):217-226.

10. Joana M, Duarte RE, Bolton JL, Huang Y, Caceres A, Veliz M, et al. Medical potential of plants used by the Q’eqchi Maya of Livingston, Guatemala for the treatment of women’s health complains. Journal of Ethnopharmacology. 2007. 114: 92-101.

11. Priyadi H, Takao G, Rahmawati I, Supriyanto B, Nursal WI, Rahman I. Five hundred plant species in Gunung Halimun Salak National Park, West Java: a checklist including sundanese names, distribution, and use. CIFOR. 2010.

12. Purwaningsih. Diversitas flora di Kawasan Koridor Taman Nasional Gunung HalimunSalak. Jurnal Teknik Lingkungan Edisi Khusus Hari Lingkungan Hidup. 2012. pp. 41-56.

13. Anonymous. Farmakope herbal Indonesia. Ed I. Ministry of Health of the Republik of Indonesia. Jakarta, Indonesia. 2008.

14. Hanani E, Soewandi SHW, Hayati, & Revita, N. Pharmacognostical and preliminary phytochemical evaluation of Cordia sebestena L. Pharmacognosy Journal. 2019. 11(5):1100-1105.

15. Stankovic MS, Neda F, Marina T, Slavica S. Total phenolic content, flavonoid concentrations and antioxidant activity, of the whole plant and plant parts extracts from Teucrium montanum L. var. montanum,
F. Supinum (L.) Reichenb.” Biotechnology and Biotechnological Equipment. 2011. 25 (1): 2222–2227.

16. Molyneux P. The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol., 2004. 26(2): 211-219.

17. Arikalang TG. Optimasi dan validasi metode analisis dalam penentuan kandungan total fenolik pada ekstrak daun gedi hijau (Abelmoschus manihot L.) yang diukur dengan spektrofotometer uv-vis. Jurnal Ilmiah Farmasi, Pharmacon. 2018. 7(3): 14– 21.

18. Salleh WMNHW.; Ahmad, F.; Khong, H.Y. Chemical composition of Piper stylosum Miq. and Piper ribesioides Wall. essential oils, and their antioxidant, antimicrobial and tyrosinase inhibition activities. Bol. Latinoam. Caribe Plantas Med. Aromat. 2014. 13: 488–497

19. Allison SD, Treseder KK. Warming and drying suppress microbial activity and carbon cycling in Boreal forest soils. Global Change Biol. 2008. 14: 2898-2909.

20. Liu WX, Zhang Z, Wan SQ. Predominant role of water in regulating soil and microbial respiration and their responses to climate change in a semiarid grassland. Global Change Biol. 2009. 15: 184-195.

21. Verma, N. Shukla, S. Impact of various factors responsible for fluctuation in plant secondary metabolites. J. Appl. Res. Med. Aromat. Plants 2015. 2: 105–113.

22. Ferrandino A. Lovisolo C. Abiotic stress effects on grapevine (Vitis vinifera L.): Focus on abscisic acid- mediated consequences on secondary metabolism and berry quality. Environ. Exp. Bot. 2014. 103: 138–147.

23. Griesser M, Weingart G, Schoedl-Hummel K, Neumann N, Becker M. et al. Severe drought stress is affecting selected primary metabolites, polyphenols, and volatile metabolites in grapevine leaves (Vitis vinifera cv. Pinot noir). Plant Physiol. Biochem. 2015. 88: 17–26 .

24. Bennett RN, Wallsgrove RM. Secondary metabolites in plant defence mechanisms. New Phytol. 1994. 127: 617–633.

25. Taylor AO. Some effects of photoperiod on the biosynthesis of phenylpropane derivatives in xanthium. plant physiol. 1965, 40, 273

26. Hashimoto T, Yamada Y. Alkaloid biogenesis: molecular aspects. Annu. Rev. Plant Biol. 1994. 45: 257–285.

27. Vincent RM, Lopez-Meyer M, McKnight TD, Nessler CL. Sustained harvest of camptothecin from the leaves of Camptotheca acuminata. J. Nat. Prod. 1997. 60: 618–619.

28. Gläßgen WE, Rose A, Madlung J, Koch, W, Gleitz J, Seitz HU. Regulation of enzymes involved in anthocyanin biosynthesis in carrot cell cultures in response to treatment with ultraviolet light and fungal elicitors. Planta 1998. 204: 490–498

29. Regvar M, Bukovnik U, Likar M, Kreft I. UV-B radiation affects flavonoids and fungal colonisation in Fagopyrum esculentum and F. tataricum. Open Life Sci. 2012. 7: 275–283

30. Warren JM, Bassman JH, Fellman JK, Mattinson DS, Eigenbrode S. Ultraviolet-B radiation alters phenolic salicylate and flavonoid composition of Populus trichocarpa leaves. Tree Physiol. 2003. 23: 527–535.

31. Hofmann RW, Swinny EE, Bloor SJ, Markham KR, Ryan KG, Campbell BD et al. Responses of nine Trifolium repens L. populations to ultraviolet-B radiation: differential flavonol glycoside accumulation and biomass production. Ann. Bot. 2000. 86: 527–537.
Published
2021-04-19
How to Cite
ANGGIA, Vivi; RINDITA, Rindita; RAMADHANY, Fauziah Ulfa. A Phytochemical and Antioxidant Evaluation of Piper hispidum Sw. Collected from Gunung Malang Village on Different Vegetation. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 19, n. 1, p. 18-24, apr. 2021. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/926>. Date accessed: 28 sep. 2021. doi: https://doi.org/10.35814/jifi.v19i1.926.
Section
Articles