Extracellular Products of Endophytic Fungi Isolate C.1.1 and C.3.3 from Cempaka Kuning Branch (Michelia champaca L.) as an Antimicrobial

  • Adefira Elisyia Juniharti Universitas Pancasila
  • Desi Nadya Aulena Universitas Pancasila
  • Novi Yantih Universitas Pancasila
  • Nur Miftahurrohmah
  • Shirly Kumala Universitas Pancasila

Abstract

Michelia champaca L. is known to have effi cacy as an antimicrobial, antioxidant, and anticancer.
In general, endophytic fungi that live in plant tissues is capable of producing secondary metabolites as their
host plant. The objective of this research is to examine the antimicrobial potential of endophytic mold isolates C.1.1 and C.3.3 from yellow cempaka branches (Michelia champaca L.). Isolates obtained from previous research, fi rst was rejuvenated on PDA. Fermentation was carried out on each isolate for 12 days. The fermented supernatant was then extracted partially with n-hexane, ethyl acetate, and n-butanol solvent. The extract was then concentrated and tested for antimicrobial activity using disk diff usion and microdilution methods. N-butanol extract of endophytic fungi isolates of yellow champaca branches was able to inhibit the microbial growth with the largest inhibition area in isolate C.1.1 in Staphylococcus aureus ATCC 6538 (12.26 mm), Escherichia coli ATCC 8739 (11.40 mm), Candida albicans ATCC 10231 (10.50 mm), and Metichillin-Resistant Staphylococcus aureus (11.30 mm) with Minimum Inhibitory Concentration and Minimum Concentration of 12,5% . It was concluded that both endophytic fungi isolates of yellow champaca branches had the potential to inhibit the growth of pathogenic microbes.

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Published
2021-05-01
How to Cite
JUNIHARTI, Adefira Elisyia et al. Extracellular Products of Endophytic Fungi Isolate C.1.1 and C.3.3 from Cempaka Kuning Branch (Michelia champaca L.) as an Antimicrobial. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 19, n. 1, p. 131-138, may 2021. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/985>. Date accessed: 20 june 2021. doi: https://doi.org/10.35814/jifi.v19i1.985.
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