Influence of Soymilk Fermentation with Lactobacillus sp. on Thin Layer Chromatography Profile and Content of Genistein and its Formulation in Effervescent Granules

  • LATIFAH RAHMAN UNIVERSITAS HASANUDDIN
  • HUSNUL WARNIDA AKADEMI FARMASI SAMARINDA
  • NATSIR DJIDE UNIVERSITAS HASANUDDIN

Abstract

lsoflavones in soybean and unfermented soybean product are in glycoside form which could not be absorbed by the body and should be hydrolyzed before it is metabolized. Hydrolysis by bacteria producing β-glucosidase occur throughout the gastrointestinal tract. Probiotic microorganisms, like Lactobacillus and bfiidobacterium, have an endogenous enzyme , β-glucosidase that plays an important role in changing the profile of isoflavones during fermentation. The study was to investigate TLC profile and genestein content in fermented soybeanmilk and to formulate the freeze-dried fermented soybeanmilk into effervescent granules preparation. Soybeanmilk was fermented with Lactobacillus sp., and then freeze-dried. The soybeanmilk, before and after fermentation, were then extracted and analyzed by TLC-scanner. The freeze-dried fermented soymilk was prepared into four formulations of effervescent granules with variations in citric acid-tartaric acid ratio. Granules were prepared by the wet method and theirs physical characteristics were evaluated. Extracted granules were analyzed by TLC-scanner. Genistein content in the fermented soymilk has increased 145,32% as compared to the unfermented soymilk. All four effervescent granules have good physical characteristics except that the moisture content were above 0.7%. Formula containing citric acidtartaric acid ratio of (1 : 1) produced effervescent granules with the best physical characteristics.

References

1. Koswara S. Susu kedelai tak kalah dengan susu sapi. Fakultas Teknologi Pertanian IPB Bogor; 2006. 2-5.
2. Breed RS, Murray EGD, Nathan SR. Bergey’s manual of determinative bacteriology. 91th ed. Baltimore: The Williams & Wilkins Company; 1994. 540.
3. Bertuzzi G. Effervescent Granulation. In Parikh DM (ed). Handbook of pharmaceutical granulation technology. Singapore: Taylor & Francis; 2005. 365~ 82.
4. U. S. Department of Agriculture, Agricultural Research Service. USDA-Iowa State University database on the isoflavone content of foods. Release 1.3 2002.
5. Summers MP. Powders and granules. In: Aulton ME. (ed). Pharmaceutics: The science of dosage form design. London: Churchill Livingstone. 1998. 300-3.
6. Ansel HC. Pengantar bentuk sediaan farmasi. Edisi 4. Terjemahan oleh Ibrahim F. Jakarta: Universitas Indonesia Press; 2008. 175-8.
7. Ennis BJ. Theory of Granulation: An engineering perspective. In: Parikh, D.M, (ed). Handbook of pharmaceutical granulation technology. Singapore: Taylor & Francis; 2005. 9, 44, 55.
8. Wells JI, Aulton ME. In: Aulton ME, editor. Pharmaceutics: The science of dosage form design. London: Churchill Livingstone; 1998. 247-8.
9. Rankell AS, Lieberman HA, Schiffmann RF. Pengeringan. Dalam: Lachman L, Lieberman H, Kanig J, editor. Teori dan praktek industri farmasi. Terjemahan oleh Siti Suyatmi. Jakarta: Penerbit Universitas Indonesia; 1986. 110-1.
10. Otieno D 0, Ashton J F, Shah N P. Evaluation of enzymic potential for biotransformation of isoflavone phytoestrogen in soymilk by Bijidobacterium animalis, Lactobacillus acidophilus and Lactobacillus casei. Food Research International. Elsevier; 2006. 394-407.
11. Donkor ON, Shah NP. Production of B-Glucosidase and hydrolysis of isofIavone phytoestrogens by Lactobacillus acidophilus, Bijidobacterium lactis, and Lactobacillus casei in soymilk. Journal of Food Science. 2008.73(1):15-20.
12. Tjay T, Rahardja K. Obat-obat penting: Khasiat, penggunaan dan efek-efek Sampingnya. Edisi V. Jakarta: Elex Media Computindo; 2002. 219-20. Staniforth JN. Particle size analysis. In: Aulton ME, editor. Pharmaceutics: The science of dosage form design. London: Churchill Livingstone; 1998. 569-70.
Published
2012-09-30
How to Cite
RAHMAN, LATIFAH; WARNIDA, HUSNUL; DJIDE, NATSIR. Influence of Soymilk Fermentation with Lactobacillus sp. on Thin Layer Chromatography Profile and Content of Genistein and its Formulation in Effervescent Granules. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 10, n. 2, p. 126-131, sep. 2012. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/258>. Date accessed: 05 nov. 2024.
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