Isolation and Identification of Bioactive Compound of Aceton Extract Garcinia lateriflora Blume Leaf



Preliminary studies have been carried out phytochemical test acetone leaf extract Garcinia
lateriflora Blume., and toxicity test against shrimp larvae (Artemia salina Leach) and murine cell line
P388. Isolates which showed the highest mortality are proceeded further for fractionation, isolation and
purification. The structure identification of active campound carriedout by analyzing the spectrum of IR ,
GC-MS (Gas chromatography mass spectrometer) and proton nuclear magnetic resonance spectrometer
(1H-NMR ) and (13C-NMR ). The results showed that the most active against the larval shrimp Artemia
salina L. with LC50 value of 18.03 ug/mL is b-asaron, IC50 of test results on cell line murine P-388 is 47.0
ug/mL. This compound was isolated first time from the plant Garcinia lateriflora BL.


1. Lawrence GHM.Taxonomy of Vascular Plants. New York: The Macmilan Company; 1955. 603-4.

2. Burkill IH, Birtwistle W, Foxworthy FW, Scrivenor JB, and. Watsan J G. A Dictionary of Economic Products of the Malay Peninsula. Vol. 1. Milbank London: Goverments of the Straits Settlemens And Federated Malay State By the Crown Agents For the Colonies; 1935. 1046-57.

3. Balasubranian K. and Rajagopalan K. Xanthones from Garcinia mangostana, Structures of BR-Xanthone A and BR-Xanthone B. Phytochemistry. 1988. 27(5): 1552-4.

4. Likhitwitauwuid K, Padungcharoen T, Mahidol C, Ruchirawat S. 7-O-Methylgarcinone E from Garcinia cowa, Phytochemistry. 1977. 45(6): 1299-1301.

5. Inuma M, H. Tosa T, Ito, T Tanaka, Riswan S. Three New Benzophenone-Xanthone Dimers from the Root of Garcinia dlcis. Chem Pharm Bull. 1996. 44(9): 1744-47.

6. Girola M, De Benardi M, Contos S, Tripodi S. Ventura P, Guarino C, Marletta M. Dose effect in lowering activity of a new dietary integrator (chitosan, Garcinia cambogia extract and chrome). Acta Toxycol Ther. 1996. 17(1): 25-40.

7. Cao S-G, Valeri HL, Wu X–H, Keng YS, Tan B. H–K, Pereira JT , Goh JH. Novel Cytotoxic Polyprenylated Xanthonoids from Garcinia gaudichaudii (Guttiferae), Tetrahedron. 1998. 54(36): 10915-24.

8. Yoshikanwa, Masayuki, Yoshizumi, Tamoy. Chemical Abstract. 2999777c.1996. 125(23).

9. Hiroyuki M, Kinoshita M, Fukuyama Y, Kodama M, Yushizawa T, Sugiura M, Nakagawa K, Tago H. Antioxidant Xanthone from Garcinia subeliptica. Phytochemistry. 1994. 36(2): 501-6.

10. Chen S–X, Wan M, Loh B–N. Active Constituens Against HIV-1 Protease from Garcinia mangostana. Planta Med. 1996. (62): 381-2.

11. Farnsworth NR. Biological and Phytochemical screening of Plant. J Pham Sci. 1996. 55(3): 225-65.

12. Meyer BN, Ferrigini NR, Putnam JE , Jacobsen LB, Nicholas D, McLaughlin JL. Planta Medica. 1992. (45): 31-59.

13. Jerry LM, Chanf C–J, Smith DL. Bioassay For the Discovery of Natural Products : An Update, Workshop On Brine Shrimp And Potato Disc Bioassay, Bandung: Universitas Bidang Ilmu Hayati Institut Teknologi Bandung. 1990. 3-6.

14. Saroyobudiono H, Hakim EH, Juliawati LD, Latip J. Trimerstilbenoid dari kulit batang Shorea rugosa. Bull Soc Prod Chem. 2006. 6, 13-8.

15. Colegate SM, Mollyneux RJ . Bioactive Natural Products, Detection, Isolation and Structural Determination, USA: CRC Press. Inc. 1993. 20-3.

16. Ito T, Akao YY i H, Ohguchi K, Matsumoto K, Tanaka T, Linuma M, Nozawa Y. Antitumor Effect of Resveratrol Oligomers Against Human Cell Cancer Lines and the Molecular Mechanism of Apoptosis Induced by Vaticanol C. Carcinogenesis. 2003-a. 24(9): 1489-97.11.
How to Cite
HARTATI, SRI; RAHMAWATI, DEDE; WIRYOWIDAGDO, SUJASWADI. Isolation and Identification of Bioactive Compound of Aceton Extract Garcinia lateriflora Blume Leaf. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 9, n. 2, p. 126-130, sep. 2011. ISSN 2614-6495. Available at: <>. Date accessed: 23 june 2024.