Penangkapan Radikal 2,2-Difenil-1-Pikril Hidrazil oleh Ekstrak Buah Psidium guajava. L dan Averrhoa carambola L.



Two fruits, Guava (Psidium guajava L.) and Carambola (Averrhoa cammbola L.), Which are frequently consumed by Indonesian people, have been evaluated for its activities as 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The fruits were macerated, evaporated, dissolved in Water, partitioned with chloroform and then with aethyl acetate to afford methanolic extract and fractions of chloroform, aethyl acetate, and Water respectively. Total phenolic and total flavonoid contents of each extract and fraction Were determined by spectrophotometric technique and used to evaluate the correlation between the natural chemical contents and their activities as DPPH radical scavengers. The results showed that extracts and fractions of R guajava L. fruit have more ability to scavenge 2,2-diphenyl- 1-picrylhidrazyl (DPPH) radical than those of A. carambola L. The total phenolic and total flavonoid contents of extracts and fractions of R guajava L. fruit are also higher than those of A. cammbola L.


1. Shui GH, Leong LP. Analysis of polyphenolics antioxidants in star fruit using liquid chromatography and mass spectrometry. J Chromatograph A. 2004. 1022:67-75.

2. Ghiselli A, Nardini M, Baldi A, Scaccini C. Antioxidant activity of different phenolics fractions separated from an Italian Red Wine. J Agric Food Chem. 1998.(46):361-7.

3. Gill Ml, Tomas FAB, Pierce BH, Kader AA. Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of Nectarine, Peach, and Plum cultivars from Califomia. J Agric Food Chem. 2002.(50):4976-82.

4. Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H. Antioxidants properties of ferulicacid and its related compounds. J Agric Food Chem. 2002. (50):2161-8.

5. Pokomi J, Yanishlieva N, Gordon M. Antioxidant in food; Practical applications. New York: CRC Press; 2001.
6. Tsai TH, Tsai PJ, Ho SC. Antioxidant andanti- inflammatory activities of several commonly used spices. J Food Sci. 2005.70 (1):C93-7.

7. Sudarsono, Gunawan D, Wahyuono S, Argodonatus I, Untoro P. Tanaman obat H: Hasil penelitian, sifat- sifat, dan penggunaannya. Yogyakarta: Pusat Studi Obat Tradisional UGM, 2002. hal. 156-60.

8. Leong LP, Sul G. An investigation of antioxidant capacity of iiuits in Singapore markets. J Food Chem. 2002.(76):69-75.

9. Morton J. Carambola: Fruit of Warm climates. Miami, Florida, USA. 1987.125-28.

10. Montoro P, Braca A, Pizza C, Tommasi ND. Structure-antioxidant activity relationship offlavonoids isolated from different plant species. .T Food Chem. 2005. (92):349-55.

11. Zou Y Lu K Wei D. Antioxidant activity of flavonoid rich extract of Hypericum pelforatum L in vitro. J Agric Food Chem. 2004.(52):5032-9.

12. Chun OK, Kim DO, Lee CY Superoxide radical scavenging activity of the maj or polyphenols in fresh 'Plums J Agric Food Chem. 2003.(51):8067-72.

13. Kim DK, Lee KW, Lee HJ, Lee CY. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J Agric Food Chem. 2002.(50): 3713-7.

14. Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of extracts containing phenolic compounds. Agric Food Chem. 1999.(47):3954-62.

15. Amic D, Amic DD, Beslo D, Trinajstic. Structure-radical scavenging activity relationship of Havonoids. Croatia Chem.Acta. 2003.76(1):55-61.

16. Mongkolsilp M, Pongbupakit I, Sae-Lee N, Sitthithaworn W. Radical scavenging activity and total phenolic content of medicinal plants used in primary health care, SWU. J Pharm Sci. 2004. (9): 32-5.

17. Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. J. Food Chem. 2003. (83): 547-50.
How to Cite
ROHMAN, ABDUL et al. Penangkapan Radikal 2,2-Difenil-1-Pikril Hidrazil oleh Ekstrak Buah Psidium guajava. L dan Averrhoa carambola L.. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 7, n. 1, p. 1-5, apr. 2009. ISSN 2614-6495. Available at: <>. Date accessed: 08 june 2023.