Uji Aktivitas Inhibisi Enzim Tirosinase In-vitro Krim Ekstrak Akar Manis (Glycyrrhiza glabra L.)
Abstrak
Ekstrak akar manis (Glycyrrhiza glabra L.) mengandung senyawa isoflavon glabridin yang dapat menghambat oksidasi l-tirosin dan L-DOPA dalam pembentukan melanin sehingga berkhasiat sebagai pemutih kulit. Tujuan penelitian menentukan potensi terbaik krim pemutih kulit menggunakan kontrol positif asam kojat. Diformulasi 1 formula blangko, 3 formula krim menggunakan variasi konsentrasi ekstrak etanol 70% akar manis (0,01, 0,11, 1,01)% berdasarkan konsentrasi inhibisi tirosinase in vitro ekstrak (IC50, 75, 100)%. Digunakan metode secara spektrofotometri dengan mengukur serapan dopakrom menggunakan microplate reader diinkubasi suhu 37°C, λ 490 nm, 20 menit. Krim M/A dibuat dengan mencampurkan ekstrak bersama basis bersuhu 70-75°C, 400 rpm, 25 menit. Uji stabilitas dilakukan 4 minggu pada penyimpanan suhu kamar dan 40°C, dievaluasi mutu fisiknya. Hasil IC50 asam kojat sebesar 20,88 µg/mL; IC50 variasi ekstrak berturut-turut sebesar (126,75; 1130,90; 10092,41) µg/mL. Krim berwarna putih susu-putih kekuningan; ber-aroma bunga; lembut; homogen; tidak terjadi pemisahan; bertipe M/A; sifat alir tiksotropik plastis; viskositas, kemampuan sebar, ukuran globul, pH semakin meningkat berturut-turut sebesar (455000- 620000)cPs, (2695,82-3545,83)mm2, (54,66-66,27)μm, (4,44-5,04); aktivitas inhibisi tirosinase formula 1, 2 dan 3 penyimpanan minggu 0, 2 dan 4 berturut-turut sebesar (1,78;-25,74;22,04)%, (6,74;6,12;4,49)%, (-28,78;53,06;20,32)%. Dapat disimpulkan formula krim dengan konsentrasi ekstrak akar manis 1,01% merupakan formula terbaik sehingga dapat digunakan sebagai pemutih kulit.
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