Anti-Inflammation Activity Test of Nanoparticles Ethanol Extract of Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) with Protein Denaturation Inhibition Method

  • Yunahara Farida Universitas Pancasila
  • Deni Rahmat Universitas Pancasila
  • Agi Widia Amanda Universitas Pancasila

Abstract

Javanese turmeric rhizome (Curcuma xanthorrhiza Roxb.) has been known to have many health benefits as antibacterial, anti-inflammatory, antidiabetic and anticancer. The purpose of this study was to compare the anti-inflammatory activity of temulawak rhizome extract with its nanoparticles. Temulawak rhizome extract was obatained by maceration using 96% (v/v) ethanol as a solvent. The extracts were made into nanoparticles by ionic gelation method which used chitosan and tripolyphosphate. The results of antiinflammatory activity test using a method of inhibiting protein denaturation showed the persentage of inhibition of protein denaturation (IC50) of the extract of temulawak rhizome of 521.67±5.80 ppm while the IC50 of the nanoparticles was 398.02±1.78 ppm. Accordingly, the antiinflammatory activity of the nanoparticles was better than the extract of temulawak rhizome.

References

1. Aggarwal S, Takada Y, Singh S, Myers JN, Aggar¬wal BB. 2004. Inhibition of growth and sur¬vival of human head and neck squamous cell carcinoma cells by curcumin via modulation of nuclear factor-kappaB signaling. Int J Cancer. 111:679–92.

2. Devaraj S, Ismail S, Ramanathan S, Yam MF. 2014. Investigation of antioxidant and hepatopro¬tective activity of standardized Curcuma xanthorrhiza rhizoma in carbon tetrachlo¬ride-induced hepatic damaged rats. The Sci¬entific World Journal. 2014;1-8.

3. Wirawan D. 2015. pengaruh nanopartikel ekstrak temulawak (curcuma xantohrriza roxb.) berbasis kitosan na-tripolofosfat dalam sediaan gel terhadap aktivitas antiacne. Skripsi Universitas Pancasila.

4. Verma MA, Kumar PA; Kavitha D, Anurag KB. 2011. Anti Denaturation and Antioxidant Activities of Annona cherimola In Vitro. International Journal of Pharma and Bio Sciences, 2(2):1-6

5. Tiwari P, Kumar B, Kaur B, Kaur G, Kau H. 2011. Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia. 1(1): 98-106.

6. Ayoola GA, Coker HAB, Adesegun SA, Bello AAA, Obaweya K1, Ezennia EC, Atangbayila TO. 2008. Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research, 7 (3): 1019-1024.

7. Williams LA, O'Connar A, Latore L, Dennis O, Ringer S, Whittaker JA, et al. 2008. The in vitro Anti-denaturation Effects Induded by Natural Products and Non-steroidal Compounds in Heat Treated (Immunogenic) Bovine Serum Albumin is Prposed as a Screening Assay for the Detection of Anti inflammatory Compounds, without the uses of Animals, in the Early Stages of the Drug Discovery Process. West Indian Med J; 57 (4): 327-330

8. Mizushima Y and Kobayashi M.1968. Interaction ofanti‐inflammatory drugs with serum preoteins, especially with some biologically active proteins. J of Pharma Pharmacol, 20:169‐ 173.

9. Leelaprakash G, Mohan Dass S. 2010. In vitro antiinflammatory activity of methanol extract of Enicostemma axillare, International Journal of Drug Development & Research 3:189-196.

10. Ingle PV, Patel DM. 2011. C-reactive protein in various disease condition – an overview, Asian J Pharm Clin Res 4(1):9-13.

11. Mann G. Chemistry of the proteids, London and New York. 1906; 336-344.

12. Robertson T B. The physical chemistry of the proteins, New York and London. 1918.

13. Chick, H, Martin, CJ. On the heat coagulation of protein.J Physiol. 1910; 4:404-430.

14. Hayun H, Arrahman A, Purwati EM, Yanuar A, Fortunata F, Suhargo F, et al.2018. Synthesis, Anti-inflammatory and Antioxidant Activity of Mannich Bases of Dehydrozingerone Derivatives. J Young Pharm10(2):6-10.
Published
2018-10-30
How to Cite
FARIDA, Yunahara; RAHMAT, Deni; AMANDA, Agi Widia. Anti-Inflammation Activity Test of Nanoparticles Ethanol Extract of Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) with Protein Denaturation Inhibition Method. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 16, n. 2, p. 225-230, oct. 2018. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/569>. Date accessed: 23 june 2024. doi: https://doi.org/10.35814/jifi.v16i2.569.
Section
Articles