Mutasi Sinar Gamma untuk Meningkatkan Kandungan Metabolit Sekunder dan Analisis Isozim Jamur Tiram

  • Ira Djajanegara Pusat Pengkajian dan Penerapan Teknologi Bioindustri
  • Retno Lestari FMIPA Departemen Biologi Universitas Indonesia
  • Harsoyo Harsoyo Badan Tenaga Atom Nasional
  • Priyo Wahyudi Pusat Pengkajian dan Penerapan Teknologi Bioindustri

Abstract

Oyster mushroom (Pleurotus ostreatus) is one of the commonly consumed mushrooms due to its nutritious as well as its pharmaceutical value. It has been known that this mushroom contains several substances such as interferon inducer to help prevent several diseases i.e. hepatitis and many others. There are three cultivars of oyster mushroom based on the color of their fruit body, namely white oyster mushroom (Pleurotus floridae), grey oyster mushroom (Pleurotus sajur-caju) and brown oyster mushroom (Pleurotus cystidious). A possibility to increase its phamaceutical value is mutation using Gamma ray. The doses of mutation used are 0.25 KGy, 0.5 KGy, 0.75 KGy, and 1.0 KGy respectively. Since most of the pharmaceutical substances are secondary metabolite products of this mushroom, the mutation is intended to increase these metabolites. In this preliminary experiment, the mutation process is observed using isozyme analysis of the mutated mycelia as the indicator.

References

1. Djarijah NM dan Djarijah AB. Budidaya jamur tiram. Yogyakarta: Penerbit Kanisius; 2001. hal. 67.

2. Busby B. Radiation and radioactivity. diambil darihttp://www.physics.isu.edu/radinf.htm. diakses 6 April, 2003.

3. Esser K. Application and importance of fungal genetics for industrial research. In: Radiation & radioisotopes for industrial microorganisms. Wina: IAEA; 1971.p. 83-91.

4. Slater R.J. Radioisotope technique. In: Wilson K and Walker J, editors. Principles and technique of practical biochemistry. 5th ed. Cambridge: Cambridge University Press, 2000. p. 687-728.

5. Holme DJ and Peck H. Analitical chemistry. 2nd ed. Burnt Mill: Longman Scientific & Technical; 1994.p. 507.

6. Hartana A. Pelatihan singkat teknik analisis dengan metode dan peraiatan muktahir di bidang hayati dan kimia. Bogor: Pusat Studi Ilmu Hayati Lembaga Penelitian IPB; hal. 76.

7. Holmes JA, Gibbs LM, Strauss HW, Mitchell M, Goff JA and Casper G. Part I: Biological effects of radiation and unit of dose. 1999; [4 hlm.]. diambil dari http:// www.stanford.edu/dept/EHS/prod/researchlab/ radlaser/manual/part1/biological.htm. diakses 4 Juni, 2003.

8. Simpkins I. General principles of biochemicals investigations. In: Wilson K. and Walker J, editors. Principles and technique of practical biochemistry. 5th ed. Cambridge: Cambridge University Press, 2000. p. 1-97.

9. SIUC (Southern Illinois University Carbondale). Starch gel electrophoresis of plant tissue. 2000. [4 hlm]. diambil dari http://www.science.siu.edu/ nickrent/PLB420/Isozyme Techniques. diakses 8 Juni, 2003.

10. Walker J. Protein structure, purification & characterization. In: Wilson K and Walker J, editors. Principles and technique of practical biochemistry. 5th ed. Cambridge: Cambridge University Press, 2000. p. 312-56.

11. Larraya LM, Perez G, Ritter E, Pisabarro AG and Ramirez G. Genetic linkage map of the edible basidiomycete Pleurotus ostreatus. Applied and Environmental Microbiology 2000.66 (12): 5290-300.

12. Otrosina WJ, Chase TE and Cobb FE. Allozymes differentiation of intersterility group of Herero
busidion annosum isolated from conifer in Western US. Phytopathology 1991.82 (5):540-45.

13. Griffiths AJF, Miller JH, Suzuki DT, Lewontin RC andGelbart WM. An introduction to genetic analysis. XVII. New York: WH Freeman; 2000. p. 860.

14. Lemont JF. Genetic and physiological factors affecting repair and mutagenesis in yeast. In: Generoso WM, Shelby MD and Deserres FJ, editors. DNA repair and mutagenesis in eukaryotes. New York: Plenum; 1980.p. 85-111.
How to Cite
DJAJANEGARA, Ira et al. Mutasi Sinar Gamma untuk Meningkatkan Kandungan Metabolit Sekunder dan Analisis Isozim Jamur Tiram. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 2, n. 1, p. 11-18, apr. 2004. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/638>. Date accessed: 24 nov. 2024.
Citation Formats
Issue
Vol 2 No 1 (2004): JIFI
Section
Articles

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.