Pertumbuhan Trichoderma harzianum pada Medium yang Mengandung Xilan

  • Priyo Wahyudi Badan Pengkajian dan Penerapan Teknologi
  • Untung Suwahyono Badan Pengkajian dan Penerapan Teknologi
  • Sri Mulyati Universitas Negeri Jakarta

Abstract

Xylan is one of the most important components of hemicelluloses, which could be enzimatically hydrolized by xylanolytic enzymes. One of the xylanase producing fungi is Trichoderma harzianum, which growth in a medium requires sufficient nutrient, such as Carbon, Nitrogen, microelements and vitamins. Xylan as a polysaccharide could be used as Carbon source in a growth medium of T. harzianum. The purpose of this experiment is to assess the growth ability of T. harzianum in a medium with xylan as a sole Carbon source. Three media are tested in this experiment, i.e. Medium I, Medium II, and Medium III. The result shows that the best growth of T. harzianum is obtained in Medium III, followed by Medium II and then Medium I. The best medium pH is neutral (+7) to mild acidic (6.2).

References

1. Utari E. Produksi dan karakteristik ensim xilanase bakteri termofilik Bacillus sp. [tesis]. Bogor: IPB; 2001. hal. 35.

2. Degrasi GBC, Okeke CV, Bruschii & Venturi V. Purification and characterization of an acetyl xylan esterase from Bacillus pumilus. Appl & Enz Microbiol. 1998;64:789-792

3. Biely P& Tenkanen N. Enzimology of hemicellulose degradation. In: Harman GE & Kubicek CP, editors. Trichoderma and Gliocladium: enzymes, biological control and commercial application. Vol 2. London: Taylor & Francis Ltd; 1998. p. 25-47.

4. Marques SL, Alves S, Ribeuro G & Amaral-Collaco MT. Characterization of a thermostable and alkalotolerant xylanase from a Bacillus sp. Appl Biochem & Biotechnol. 1998;73:159-172.

5. Cortez EV, Pessoa A, Jr & Assis AN. Xylanase recovery by ethanol and Na, SO, precipitation. Appl Boichem & Biotechnol. 1998;70-72:660-666.

6. Buchert JT, Oksanen J, Pere M., Siika – Aho A., Suurnakki & Viikari L. Application of T. reesei enzymes in the pulp and paper industri. In: Harman GE & Kubicek CP, editors. Trichoderma and Gliocladium: enzymes, biological control and commercial application. Vol 2. London: Taylor & Francis Ltd.; 1998. p. 343-363.

7. Breccia JD, Torto N, Gorton L, Sineriz F & Hatti-Kaul R. Specifity and mode of action of thermostable xylanase from Bacillus amyloliquefaciens on – line monitoring of hydrolysis products. Appl Biochem & Biotechnol. 1998;69:31-39.

8. Klein D & Eveleigh DE. Ecology of Trichoderma. In: Harman GE & Kubicek CP, editors. Trichoderma and Gliocladium: basic biology, taxonomy & genetics. Vol 1. London: Taylor & Prancis Ltd.; 1998. p. 57-69.

9. Suwahyono U & Wahyudi P. Trichoderma harzianum dan aplikasinya. Jakarta: Direktorat Teknologi Bioindustri BPPT; 2000.

10. Nakamura SK, Wakabayashi R, Nakai R, Aono & Horikoshi K. Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41 M-1. Appl Environ Microbiol. 1993;59(7):2311-2316.

11. Tripanji & Siswanto. Production of chitinase and cellulase from Trichoderma harzianum cocoa sweating as a culture medium. Prosiding Seminar Kimia II; 1999; Yogyakarta; UGM. 1999.

12. Yabuki M. Characterization of chitosanase produced by Baccilius circulans MH-KI. London: Elsevier applied science; 1989.

13. Fardiaz S. Mikrobiologi pangan. Jakarta: Gramedia Pustaka Utama; 1992. 14. Atlas RM. Principles of microbiology. Iowa: Brown Publishers; 1997.
How to Cite
WAHYUDI, Priyo; SUWAHYONO, Untung; MULYATI, Sri. Pertumbuhan Trichoderma harzianum pada Medium yang Mengandung Xilan. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 2, n. 1, p. 31-36, apr. 2004. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/641>. Date accessed: 05 nov. 2024.
Citation Formats
Issue
Vol 2 No 1 (2004): JIFI
Section
Articles

Licencing
All articles in Jurnal Ilmu Kefarmasian Indonesia are an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License which permits unrestricted non-commercial used, distribution and reproduction in any medium.

This licence applies to Author(s) and Public Reader means that the users mays :

  • SHARE:
    copy and redistribute the article in any medium or format
  • ADAPT:
    remix, transform, and build upon the article (eg.: to produce a new research work and, possibly, a new publication)
  • ALIKE:
    If you remix, transform, or build upon the article, you must distribute your contributions under the same license as the original.
  • NO ADDITIONAL RESTRICTIONS:
    You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

It does however mean that when you use it you must:

  • ATTRIBUTION: You must give appropriate credit to both the Author(s) and the journal, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.

You may not:

  • NONCOMMERCIAL: You may not use the article for commercial purposes.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.