Pertumbuhan Trichoderma harzianum pada Medium yang Mengandung Xilan

  • Priyo Wahyudi Badan Pengkajian dan Penerapan Teknologi
  • Untung Suwahyono Badan Pengkajian dan Penerapan Teknologi
  • Sri Mulyati Universitas Negeri Jakarta

Abstract

Xylan is one of the most important components of hemicelluloses, which could be enzimatically hydrolized by xylanolytic enzymes. One of the xylanase producing fungi is Trichoderma harzianum, which growth in a medium requires sufficient nutrient, such as Carbon, Nitrogen, microelements and vitamins. Xylan as a polysaccharide could be used as Carbon source in a growth medium of T. harzianum. The purpose of this experiment is to assess the growth ability of T. harzianum in a medium with xylan as a sole Carbon source. Three media are tested in this experiment, i.e. Medium I, Medium II, and Medium III. The result shows that the best growth of T. harzianum is obtained in Medium III, followed by Medium II and then Medium I. The best medium pH is neutral (+7) to mild acidic (6.2).

References

1. Utari E. Produksi dan karakteristik ensim xilanase bakteri termofilik Bacillus sp. [tesis]. Bogor: IPB; 2001. hal. 35.

2. Degrasi GBC, Okeke CV, Bruschii & Venturi V. Purification and characterization of an acetyl xylan esterase from Bacillus pumilus. Appl & Enz Microbiol. 1998;64:789-792

3. Biely P& Tenkanen N. Enzimology of hemicellulose degradation. In: Harman GE & Kubicek CP, editors. Trichoderma and Gliocladium: enzymes, biological control and commercial application. Vol 2. London: Taylor & Francis Ltd; 1998. p. 25-47.

4. Marques SL, Alves S, Ribeuro G & Amaral-Collaco MT. Characterization of a thermostable and alkalotolerant xylanase from a Bacillus sp. Appl Biochem & Biotechnol. 1998;73:159-172.

5. Cortez EV, Pessoa A, Jr & Assis AN. Xylanase recovery by ethanol and Na, SO, precipitation. Appl Boichem & Biotechnol. 1998;70-72:660-666.

6. Buchert JT, Oksanen J, Pere M., Siika – Aho A., Suurnakki & Viikari L. Application of T. reesei enzymes in the pulp and paper industri. In: Harman GE & Kubicek CP, editors. Trichoderma and Gliocladium: enzymes, biological control and commercial application. Vol 2. London: Taylor & Francis Ltd.; 1998. p. 343-363.

7. Breccia JD, Torto N, Gorton L, Sineriz F & Hatti-Kaul R. Specifity and mode of action of thermostable xylanase from Bacillus amyloliquefaciens on – line monitoring of hydrolysis products. Appl Biochem & Biotechnol. 1998;69:31-39.

8. Klein D & Eveleigh DE. Ecology of Trichoderma. In: Harman GE & Kubicek CP, editors. Trichoderma and Gliocladium: basic biology, taxonomy & genetics. Vol 1. London: Taylor & Prancis Ltd.; 1998. p. 57-69.

9. Suwahyono U & Wahyudi P. Trichoderma harzianum dan aplikasinya. Jakarta: Direktorat Teknologi Bioindustri BPPT; 2000.

10. Nakamura SK, Wakabayashi R, Nakai R, Aono & Horikoshi K. Purification and some properties of an alkaline xylanase from alkaliphilic Bacillus sp. strain 41 M-1. Appl Environ Microbiol. 1993;59(7):2311-2316.

11. Tripanji & Siswanto. Production of chitinase and cellulase from Trichoderma harzianum cocoa sweating as a culture medium. Prosiding Seminar Kimia II; 1999; Yogyakarta; UGM. 1999.

12. Yabuki M. Characterization of chitosanase produced by Baccilius circulans MH-KI. London: Elsevier applied science; 1989.

13. Fardiaz S. Mikrobiologi pangan. Jakarta: Gramedia Pustaka Utama; 1992. 14. Atlas RM. Principles of microbiology. Iowa: Brown Publishers; 1997.
How to Cite
WAHYUDI, Priyo; SUWAHYONO, Untung; MULYATI, Sri. Pertumbuhan Trichoderma harzianum pada Medium yang Mengandung Xilan. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 2, n. 1, p. 31-36, apr. 2004. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/641>. Date accessed: 27 apr. 2024.
Section
Articles