Effect of Extraction Method on Polyphenol Content and Antioxidant Activity of Rubus fraxinifolius

  • Yesi Desmiaty Fakultas Farmasi Universitas indonesia dan Universitas Pancasila
  • Berna Elya Fakultas Farmasi Universitas Indonesia
  • Fadlina Chany Saputri Fakultas Farmasi Universitas Indonesia
  • Iis Irawatty Dewi Fakultas Farmasi Universitas Pancasila
  • Muhammad Hanafi Pusat Penelitian Kimia LIPI, Serpong dan FAkultas Farmasi Universitas Pancasila


Rubus fraxinifolius can be founded in the mountainous regions of West Java. Previous studies have shown that this plant has potent antioxidant activity. The content of polyphenol compounds in this species contributes substantially to antioxidant activity, and the extraction method will affect the content of the compound. In this study an evaluation of the extraction method effect on leaves, young fruits and stems of R. fraxinifolius on the content of polyphenols and their antioxidant activity, have been done. Method: The leaves, fruits, and stems of R. fraxinifolius were extracted by maceration, reflux, and soxhlet using methanol as a solvent. Each extract was determined the total polyphenol content using Folin-Ciocalteu reagent and antioxidant activity test using DPPH reduction method. Result: The highest extract yield was R. fraxinifolius leaf reflux extract (30.20%). The highest total polyphenol content was given by the Soxhlet extract of leaf R. fraxinifolius (48.79 mg GAE / g extract). The highest antioxidant activity was leaf Soxhlet extract of R. fraxinifolius (98.29% at 100 ppm). Conclusion: From this study it was shown that the methanol extract of R. fraxinifolius leaves extracted by Soxhlet gave the highest polyphenol content and antioxidant activity.


1. Kalkman C, Verheij EWM, Coronel R. Rubus [Internet]. PROSEA (Plant Resources of South-East Asia) Foundation. 1991 [cited 2019 Sept 8]. Available from: http://proseanet.org/prosea/e-prosea_detail.php?frt=e&id=8

2. Surya MI, Suhartati S, Ismaini L, Lusini Y, Destri D, Anggraeni D, et al. Fruit Nutrients of Five Species of Wild Raspberries (Rubus spp.) from Indonesian Mountain’s Forests. J Trop Life Sci. 2018;Jan 15;8(1):75–80.

3. Bakar MFA, Ismail NA, Isha A, Ling ALM. Phytochemical Composition and Biological Activities of Selected Wild Berries (Rubus moluccanus L., R. fraxinifolius Poir., and R. alpestris Blume). Evidence-Based Complement Altern Med. 2016;2016(November):1–10.

4. Shamsudin NA, Matawali A, Azlan J. Comparison of Antioxidant Activity and Phytochemical Content of Borneo Wild Berry, Rubus fraxinifolius (Rogimot). Vol. 6, Transactions on Science and Technology. 2019.

5. Galvez MAC. Evaluation of DPPH Free Radical Scavenging Activity and Phytochemical Screening of Selected Folkloric Medicinal Plants in Tinoc, Ifugao, Cordillera Administrative Region, Philippines. Int J Sci Res Publ. 2014;5(12).

6. Desmiaty Y, Elya B, Saputri FC, Hanafi M, Prastiwi R. Antioxidant Activity of Rubus fraxinifolius Poir. and Rubus rosifolius J. Sm. Leaves. J Young Pharm. 2018;10(2s):S93–6.

7. Bobo-García G, Davidov-Pardo G, Arroqui C, Vírseda P, Marín-Arroyo MR, Navarro M. Intra-laboratory validation of microplate methods for total phenolic content and antioxidant activity on polyphenolic extracts, and comparison with conventional spectrophotometric methods. J Sci Food Agric. 2015;95(1):204–9.

8. Ignat I, Volf I, Popa VI. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem. 2011;126(4):1821–35.

9. Karim AA, Azlan A, Ismail A, Hashim P, Abd Gani SS, Zainudin BH, et al. Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract. BMC Complement Altern Med. 2014;14(1):381.

10. Barcelo R. Phytochemical Screening and Antioxidant Activity of Edible Wild Fruits in Benguet , Cordillera Administrative Region , Philippines. Electron J Biol. 2015;11(3):80–9.

11. Mojzer EB, SKerget M, Knez Z, Bren U. Polyphenols: Extraction methods, antioxidative action, bioavailability and anticarcinogenic effects. Molecules. 2016;21(7).

12. Pientaweeratch S, Panapisal V, Tansirikongkol A. Antioxidant, anti-collagenase and anti-elastase activities of Phyllanthus emblica , Manilkara zapota and silymarin: an in vitro comparative study for anti-aging applications. Pharm Biol. 2016;54(9):1865–72.

13. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011;48(4):412–22.

14. Fu L, Xu BT, Xu XR, Qin XS, Gan RY, Li H Bin. Antioxidant capacities and total phenolic contents of 56 wild fruits from South China. Molecules. 2010;15(12):8602–17.

15. Poulin RC, Sasikumar JM, Meseret CE, Selvakumar P. In vitro analysis of antioxidant capacity of Indian yellow raspberry (Rubus ellipticus Smith.). International Food Research Journal. 2015; 22(4): 1338-1346
How to Cite
DESMIATY, Yesi et al. Effect of Extraction Method on Polyphenol Content and Antioxidant Activity of Rubus fraxinifolius. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 17, n. 2, p. 227-231, oct. 2019. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/755>. Date accessed: 18 nov. 2019. doi: https://doi.org/10.35814/jifi.v17i2.755.