Development of Simple High Performance Liquid Chromatography for Analysis of Curcumin in Human Plasma In-Vitro

  • Novi Yantih UNIVERSITAS PANCASILA
  • Endah Giyah Wahyuningsih Universitas Pancasila
  • Deni Rahmat Universitas Pancasila
  • Yunahara Farida Universitas Pancasila
DOI: https://doi.org/10.35814/jifi.v18i2.947

Abstract

Curcumin has antioxidant, antiinfl ammatory, antitumor, apoptotic-inducing, and
antiangiogenesis eff ects. In order to study the pharmacokinetics of curcumin, a method for analysis
of curcumin in plasma levels is required. The aim of this study was developing of a simple High
Performance Liquid Chromatography (HPLC) for analysis curcumin in human plasma in-vitro. The
HPLC system was using isocratic technique in column reversed phase of C18 (Reliant® RP-18e
column (4.6x250 mm; 5 μm)) and mobile phase of acetonitrile–acetic acid–aquabidest (60:1:39) at fl ow
rate of 1.0 mL/min. Irbesartan was used as an internal standard. Detector was performed at a wavelength
of 428 nm for curcumin and 270 nm for irbesartan. Linearity test shown linear results with a correlation
coeffi cient (r) of 0.9970. LLOQ value was 0.0196 μg/mL with a diff erentiation value of 10.48-18.09%.
The accuracy and precision of this method met requirement with a diff erentiation value and relative
standard deviation of between -5.51-3.04 % and 1.04-1.86 %, respectively. Recovery of this method was
94.74-103.12%. This method provides good selectivity for the analysis curcurmin in human plasma.
The developed of HPLC was a simple and valid method for analysis curcumin in human plasma.

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Published
2020-10-28
How to Cite
YANTIH, Novi et al. Development of Simple High Performance Liquid Chromatography for Analysis of Curcumin in Human Plasma In-Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 2, p. 227-234, oct. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/947>. Date accessed: 19 apr. 2024. doi: https://doi.org/10.35814/jifi.v18i2.947.
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Vol 18 No 2 (2020): JIFI
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