Development of Simple High Performance Liquid Chromatography for Analysis of Curcumin in Human Plasma In-Vitro

  • Novi Yantih UNIVERSITAS PANCASILA
  • Endah Giyah Wahyuningsih Universitas Pancasila
  • Deni Rahmat Universitas Pancasila
  • Yunahara Farida Universitas Pancasila

Abstract

Curcumin has antioxidant, antiinfl ammatory, antitumor, apoptotic-inducing, and
antiangiogenesis eff ects. In order to study the pharmacokinetics of curcumin, a method for analysis
of curcumin in plasma levels is required. The aim of this study was developing of a simple High
Performance Liquid Chromatography (HPLC) for analysis curcumin in human plasma in-vitro. The
HPLC system was using isocratic technique in column reversed phase of C18 (Reliant® RP-18e
column (4.6x250 mm; 5 μm)) and mobile phase of acetonitrile–acetic acid–aquabidest (60:1:39) at fl ow
rate of 1.0 mL/min. Irbesartan was used as an internal standard. Detector was performed at a wavelength
of 428 nm for curcumin and 270 nm for irbesartan. Linearity test shown linear results with a correlation
coeffi cient (r) of 0.9970. LLOQ value was 0.0196 μg/mL with a diff erentiation value of 10.48-18.09%.
The accuracy and precision of this method met requirement with a diff erentiation value and relative
standard deviation of between -5.51-3.04 % and 1.04-1.86 %, respectively. Recovery of this method was
94.74-103.12%. This method provides good selectivity for the analysis curcurmin in human plasma.
The developed of HPLC was a simple and valid method for analysis curcumin in human plasma.

References

1. Aggarwal BB. Curcumin-Biological And Medicinal Properties dalam Turmeric The Genus Curcuma. USA : CRC Press ; 2007.

2. Yallapu MM, Jaggi M, Chauhan SC. Curcumin Nanoformulations A Future Nanomedicine For Cancer. Drug Discov Today; 2012.

3. Parwa B. Analisis Farmasi Metode Modern. Surabaya : Airlangga University Press; 1991.

4. United States Pharmacopeial Convention. The United States Pharmacopeial 32 – The National Formulary 27. Rockville : United States Pharmacopeial Convention; 2009.

5. Committee For Medicinal Product For Human Use. Guideline On Bioanalytical Method Validatioin. European Medicines Agency; London;2011

6. Food and Drug Administration. Guidance For Industry On Bioanalytical Method Validation, U.S. Department of Health and Human Services. May 2018. Diambil dari https://www.fda.gov/ downloads/drugs/guidances/ucm070107.pdf Diakses pada 24 November 2018

7. Adawiyah L. Validasi metode analisis kurkumin dalam plasma in vitro secara kromatografi cair kinerja tinggi (skripsi), Depok: Fakultas Farmasi Universitas
Indonesia; 2011.

8. Cahyono B, Muhammad D, Leenawaty L. Pengaruh Proses Pengeringan Rimpang Temulawak (Curcuma xanthorriza Roxb) terhadap kandungan dan komposisi kurkuminoid. 2011.

9. Dhuhania CE. Optimasi dan Validasi Metode Penetapan Kadar Kurkumin Dalam Plasma Manusia (in-vitro) Secara Kromatografi Cair Kinerja Tinggi(tesis). Yogyakarta: Univeristas Gadjah Mada, 2012.

10. Ghalandarlaki N, Alizadeh AM, Ashkani-Esfahani S. Nanotechnology Applied Curcumin for Different Disease Therapy. Biomed Research International, 2014. h 1-23.

11. Departemen Kesehatan Republik Indonesia. Farmakope Indonesia Edisi V. Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan; 2014

12. Widjaja M. Validasi Metode Penetapan Kadar Kurkumin Dalam Sediaan Cair Obat Herbal Terstandar Merk Kiranti® Secara Kromatografi Cair Kinerja Tinggi Fase Terbalik (skripsi), Yogyakarta : Universitas Sanata Dharma; 2011.
Published
2020-10-28
How to Cite
YANTIH, Novi et al. Development of Simple High Performance Liquid Chromatography for Analysis of Curcumin in Human Plasma In-Vitro. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 18, n. 2, p. 227-234, oct. 2020. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/947>. Date accessed: 02 dec. 2020. doi: https://doi.org/10.35814/jifi.v18i2.947.
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Articles