Optimization of Cocktail Composition and Up-scale Fermentation Process Development of Four Skin Commensal Bacterial Strains

  • Ahmad Baikuni . Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, 16424, Indonesia
  • Meidy Richky Wanyodiharjo . Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia
  • Harry Ardiansyah . Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia
  • Fathan Luthfi Hawari . Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia
  • Amarila Malik . Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Universitas Indonesia
DOI: https://doi.org/10.35814/jifi.v20i1.1224

Abstract

Analyzing the actual microbial composition in the skin microbiome is riveting to create a potential source
of active substances in skincare products. Our previous study isolated four bacterial strains from Javanese male and female skin samples, Staphylococcus hominis MBF12–19J, Staphylococcus warneri MBF02–19J, Bacillus subtilis MBF10–19J, and Micrococcus luteus MBF05–19J. This study aimed to determine the composition of those strains in a bacterial cocktail and optimize the condition for up-scale laboratory production of the cocktail. To assess the ability of bacteria to coexist and live in cocktail communities, simultaneous monitoring was performed using the Deferred Growth Inhibition Assay (DGIA) method and real-time PCR for DNA copy number measurement. Results showed the best composition based on even distribution and cell growth viability was at the ratio 1.5:1:0.5:0.5 of Micrococcus luteus MBF05-19J, Bacillus subtilis MBF10-19J, Staphylococcus warneri MBF02-19J, Staphylococcus hominis MBF12-19J, which is equivalent to DNA copy number/mL 1.209 x 1024 CFU : 2.484 x 1041 CFU : 2.645 x 1041 CFU : 9.041 x 1035 CFU, respectively. The optimum growth incubation time of individual bacterial cultures for an up-scale 2-L bio-fermentor mixture was as follows; Micrococcus luteus MBF05-19J = 21 hrs; Bacillus subtilis MBF10-19J = 7 hrs; Staphylococcus warneri MBF02-19J = 17 hrs; Staphylococcus hominis MBF12-19 = 15 hrs. For up-scale conditions, the fermentation incubation time was 3 hours at 37oC, agitation 50 RPM, and aeration 5% dissolved oxygen.

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Published
2022-04-30
How to Cite
BAIKUNI, Ahmad et al. Optimization of Cocktail Composition and Up-scale Fermentation Process Development of Four Skin Commensal Bacterial Strains. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 1, p. 113-119, apr. 2022. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/1224>. Date accessed: 25 apr. 2024. doi: https://doi.org/10.35814/jifi.v20i1.1224.
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