Uji Sitotoksik Hasil Fermentasi Kapang Endofit Buah Mahkota Dewa (Phaleria macrocarpa) terhadap Sel MCF-7

  • Syarmalina Syarmalina Universitas Pancasila
  • Wan Lely Universitas Pancasila
  • Nazliah Laupa Universitas Pancasila

Abstract

In the development of traditional medicine, there are great demands of medicinal plant. Meanwhile, those demands exceed the capacity of natural resource to provide the raw material. The solution for that problem that has been developed is by utilizing organism that live on plant tissue, which known as endophytic microbe. The preliminary Brine Shrimp Lethality Test based research on the polarity degree of supernatant extracts has proved that it has high toxic activity on Artemia salina Leach. Subsequent to that study, a research to examine in vitro cytotoxic effect of supernatant extract fermented from Mahkota Dewa fruit endophytic mould KVM/a and KIVM/b isolate on breast cancer cell has been conducted. Thin layer chromatographic test was performed to identify the patch pattern from each fraction of KVM/a and KIVM/b isolate. Furthermore, preparative thin layer chromatographic test was tested on MCF-7 cell by using neutral red method. The cytotoxic effect was determined based on ICs, value. The results confirm the cytotoxic test effect of semi polar fraction of both isolates on MCF-7 cell with the ICs, value of 36.26 ppm for KVM/a and 30.48 ppm for KIVM/b.

References

1. Murray RK, Granner DK, Mayes PA, Rodwell VW. Biokimia harper. Edisi 22. Diterjemahkan oleh Hartono Andry. Jakarta: Penerbit Buku Kedokteran EGC; 1995. hal. 791-3, 796.

2. Tapan Erik. Kanker, antioksidan dan terapi komplementer. Jakarta: Elex Media Komputindo; 2005. hal. 40-61.

3. Sugiyanto, Sudarto, Edy M, Agung EN. Aktivitas anti karsinogenik senyawa yang berasal dari tumbuhan. Majalah Farmasi Indonesia 2003;14(3):132-41.

4. Harmanto N. Mahkota dewa: Obat pusaka para dewa. Jakarta: Agromedia Pustaka; 2005. hal. 9-13.

5. Maksum. R. Peranan bioteknologi dan mikroba endofit dalam pengembangan obat herbal. Jurnal farmasi ui [serial online]. Desember 2005;2(3):11326. Diambil dari: http://www.jurnal.farmasi.ui.ac. id/pdf/2005/vo2no3/maksum0203%5B1%5D.pdf. Diakses 24 Februari, 2006.
6. Petrini 0, Sieber TN, Toti L, Viret 0. Ecology, metabolite production, and substrate utilization in
endophytic fungi. Natural Toxins 1992;1:185-96.

7. Pengakuan untuk pemburu mikroba. Diambil dari: http://www.lipi.go.id/www/www.cgi? baca&1077973061.htm. Diakses 1 Maret, 2006.

8. Wahyudi P. Isolasi mikroorganisme endofitik tanaman tropis Indonesia. Laporan Teknis. Jakarta:BPPT 1997; 1-6.

9. Wulandari AS. Uji pendahuluan metabolit sekunder kapang endofit buah mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) dengan metode BSLT (Brine Shrimp Lethality Test) [skripsi]. Jakarta: Fakultas Farmasi Universitas Pancasila; 2005.

10. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Ni chols DE, Mclaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Drug Information Journal 1982;45:31-4.

11. Verawati. Uji toksisitas supernatan hasil fermentasi isolat KVM/a dan KIVM/b kapang endofit buah mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl.) secara Brine Shrimp Lethality Test (BSLT) [Skripsi). Jakarta: Fakultas Farmasi Universitas Pancasila; 2006.

12. Darling DC, Morgan SJ. Animal cells culture and media essential data. Chichester: John Willey and Sons; 1994.

13. Freshney I. Culture of animal cells: A manual of basic technique. 3rd ed. New York: Wiley-Liss Inc; 1994. hal. 85-8, 154-5, 260-70, 287.

14. Wilson AP. Cytotoxicity and viability assays. Dalam: Masters JRW, editor. Animal cell culture: A practical approach. 3rd ed. New York: Oxford University Press; 2000. hal. 263, 264, 272.

15. Mans Denis RA, Adriana B Da Rocha, Gilberto S. Anticancer drugs discovery and development. Targeted plants collection as a rational strategy to acquire candidate anticancer compound.
Published
2007-04-17
How to Cite
SYARMALINA, Syarmalina; LELY, Wan; LAUPA, Nazliah. Uji Sitotoksik Hasil Fermentasi Kapang Endofit Buah Mahkota Dewa (Phaleria macrocarpa) terhadap Sel MCF-7. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 5, n. 1, p. 23-30, apr. 2007. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/584>. Date accessed: 05 nov. 2024.
Section
Articles