Determination of Vitamin E Levels in n-Hexane Extract of Palm Fruits (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC Method

  • Mustika Furi Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau)
  • Rahma Dona Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau)
  • Vincent Tristan Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau)

Abstract

Palm fruit (Elaeis guineensis Jacq) is a natural source of vitamin E (tocopherol). This study has determined vitamin E levels in n-hexane extracts of palm fruit (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC (High Performance Liquid Chromatography) method. The subject were palm fruit and CPO obtained from plantations and palm fruit mills PT X located in Kampar district, Riau. Extraction of palm fruit and CPO using ultrasonication and saponification method. Measurement using HPLC obtained optimum conditions using mobile phase acetonitrile: water (86:14), flow 1 mL/minute, UV-Vis detector at wavelength 284 nm and obtained retention time is 3,488 minutes.  Determination of the calibration curve is obtained the regression equation y = 702,872x + 3273,26 and r2 = 0,9985. The result of the determination vitamin E levels in the palm fruit extract and CPO sequentially is (469,4008 ± 2,5879) µg/mg and for CPO is (262,5248 ± 6,3987) µg/mg.

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Published
2022-04-29
How to Cite
FURI, Mustika; DONA, Rahma; TRISTAN, Vincent. Determination of Vitamin E Levels in n-Hexane Extract of Palm Fruits (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC Method. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 1, p. 49-54, apr. 2022. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/915>. Date accessed: 02 july 2022. doi: https://doi.org/10.35814/jifi.v20i1.915.
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Articles