Determination of Vitamin E Levels in n-Hexane Extract of Palm Fruits (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC Method

  • Mustika Furi Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau)
  • Rahma Dona Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau)
  • Vincent Tristan Sekolah Tinggi Ilmu Farmasi Riau (STIFAR Riau)
DOI: https://doi.org/10.35814/jifi.v20i1.915

Abstract

Palm fruit (Elaeis guineensis Jacq) is a natural source of vitamin E (tocopherol). This study has determined vitamin E levels in n-hexane extracts of palm fruit (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC (High Performance Liquid Chromatography) method. The subject were palm fruit and CPO obtained from plantations and palm fruit mills PT X located in Kampar district, Riau. Extraction of palm fruit and CPO using ultrasonication and saponification method. Measurement using HPLC obtained optimum conditions using mobile phase acetonitrile: water (86:14), flow 1 mL/minute, UV-Vis detector at wavelength 284 nm and obtained retention time is 3,488 minutes.  Determination of the calibration curve is obtained the regression equation y = 702,872x + 3273,26 and r2 = 0,9985. The result of the determination vitamin E levels in the palm fruit extract and CPO sequentially is (469,4008 ± 2,5879) µg/mg and for CPO is (262,5248 ± 6,3987) µg/mg.

References

1. RPahan I. Panduan Lengkap Kelapa Sawit. Penebar Swadaya. Jakarta: Penebar Swadaya; 2011. 68, 78, 224-229. p.

2. Siregar HA, Rahmadi HY, Wening S, Suprianto E. Komposisi asam lemak dan karoten kelapa sawit Elaeis oleifera, interspesifik hibrida, dan Pseudo-backcross pertama di Sumatra Utara, Indonesia. J Penelit Kelapa Sawit. 2018 Aug 1;26(2):91-101.

3. Nor Azman NH, Goon JA, Abdul Ghani SM, Hamid Z, Wan Ngah WZ. Comparing palm oil, tocotrienol-rich fraction and α-tocopherol supplementation on the antioxidant levels of older adults. Antioxidants. 2018 Jun;7(6):74.

4. Depkes RI. Farmakope Indonesia edisi IV. Departemen Kesehatan Republik Indonesia. 1995.

5. Masykur M. Pengembangan Industri Kelapa Sawit sebagai Penghasil Energi Bahan Bakar Alternatif dan Mengurangi Pemanasan Global. J Reformasi. 2013;3(2): 96–107.

6. Vachiraanan K, Rattanapisit J, Sridang P. Hybrid Processes for Extraction-separation of Tocopherols from Crude Palm Oil. J Appl Membr Sci Technol. 2008;8(1):15-20

7. Andulaai AM, Ruslan R, Ys. H, Puspitasari DJ. Studi Perbandingan Analisis Vitamin E Minyak Sawit Merah Tersaponifikasi Antara Metode Spektrofotometri UV-Vis dan KCKT. Kovalen. 2017;3(1):50.

8. Irías-Mata A, Stuetz W, Sus N, Hammann S, Gralla K, Cordero-Solano A, Vetter W, Frank J. Tocopherols, tocomonoenols, and tocotrienols in oils of costa rican palm fruits: a comparison between six varieties and chemical versus mechanical extraction. . J Agri Food Chem. 2017 Aug 30;65(34):7476-82.

9. Martha SA, Karwur FF, Rondonuwu FS. Metode Purifikasi Vitamin E dari Minyak Kelapa Sawit. InProceeding Biology Education Conference: Biology, Science, Enviromental, and Learning 2012 (Vol. 10, No. 1).

10. Wonorahardjo S. Metode-Metode Pemisahan Kimia. Jakarta: PT Indeks; 2016. 120–121 p.

11. Gandjar IG, Rohman A. Kimia Analisis Farmasi. Pustaka Pelajar. 2007. 406 p.

12. Departemen Kesehatan Repubik Indonesia. Farmakope Indonesia Edisi V. Direktorat Jenderal Pengawasan Obat dan Makanan. 2014.

13. Harmita H. Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Maj Ilmu Kefarmasian. 2004;117–35.

14. Damanik M, Murkovic M. The stability of palm oils during heating in a rancimat. European Food Research and Technology. 2018 Jul;244(7):1293-9.

15. Setyamidjaja D. Budi Daya Kelapa Sawit. Kanisius. 2006. p. 61–3.
Published
2022-04-29
How to Cite
FURI, Mustika; DONA, Rahma; TRISTAN, Vincent. Determination of Vitamin E Levels in n-Hexane Extract of Palm Fruits (Elaeis guineensis Jacq) and CPO (Crude Palm Oil) using HPLC Method. JURNAL ILMU KEFARMASIAN INDONESIA, [S.l.], v. 20, n. 1, p. 49-54, apr. 2022. ISSN 2614-6495. Available at: <http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/915>. Date accessed: 22 dec. 2024. doi: https://doi.org/10.35814/jifi.v20i1.915.
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Vol 20 No 1 (2022): JIFI
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